製品の概要

  • 製品名Anti-Dab1 antibody
    Dab1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Dab1
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Recognizes Dab1 of 48 kDa, corresponding to amino acids 399-555 of mouse Dab1 fusion protein.

  • ポジティブ・コントロール
    • WB: 293 cells transfected with Dab1–HA tag IP: Dab1 Knockout brain p14 lysate and Dab1 Wild Type brain p14 lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 67 kDa).
IP Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Adapter molecule functioning in neural development. May regulate SIAH1 activity.
  • 配列類似性Contains 1 PID domain.
  • ドメインThe PID domain specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins.
  • 翻訳後修飾Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons (By similarity). Also phosphorylated on Ser-524 independently of reelin signaling.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dab 1 antibody
    • Dab reelin signal transducer 1 antibody
    • Dab, reelin signal transducer, homolog 1 (Drosophila) antibody
    • Dab1 antibody
    • DAB1_HUMAN antibody
    • Disabled homolog 1 antibody
    • Disabled homolog 1 Drosophila antibody
    • Scm antibody
    • Scr antibody
    • Scrambler antibody
    • Yot antibody
    • Yotari antibody
    see all

Anti-Dab1 antibody 画像



  • Predicted band size : 67 kDa


    ab16675 at 1µg/ml staining mouse Dab1 (80kDa) in mouse brain extract by Western blot. Lane 1: ab16674 (N terminal). Lane 2: ab16675 (C terminal).

Anti-Dab1 antibody (ab16675) 使用論文

ab16675 has not yet been referenced specifically in any publications.

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Thank you for contacting us yesterday in regards to antibodies to be used in immunoprecipitation of Dab1 from mouse and human samples.

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Neuroblastoma Cells)
Loading amount 400000 cells
Specification Neuroblastoma Cells
Gel Running Conditions Reduced Denaturing (Gel 10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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投稿 Mar 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"