製品の概要

  • 製品名DAB substrate kit
    DAB substrate kit キット 製品一覧
  • 製品の概要

    This product is available in a two component form consisting of a liquid, refrigerator stable DAB Chromogen and DAB Substrate. Once the two components are combined, the reagent can be used for up to six hours, making it ideal for automated stainers.

  • 特記事項

    Contains DAB in buffer. Reagent is acidic and can cause burns if skin contact occurs.

    Contains Hydrogen Peroxide in DAB substrate buffer. Avoid contact with skin and eyes.

  • アプリケーション適用あり: IHC-Pmore details

製品の特性

  • 関連性3,3' Diaminobenzidine (DAB) is a widely used chromogen for immunohistochemical staining. In the presence of peroxidase enzyme, DAB produces a brown precipitate that is insoluble in alcohol.
  • 別名
    • 3 3' Diaminobenzidine

アプリケーション

Our Abpromise guarantee covers the use of ab94665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent dilution.

DAB substrate kit 画像

  • Human colon carcinoma stained with anti-p53 antibody using DAB Substrate Kit (ab94665).

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DAB substrate kit (ab94665) 使用論文

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Abreviews
This DAB substrate kit (ab94665) is easy to use, and the quality is good.
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Abcam user community

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投稿 Aug 09 2016

Abreview for ab94665 - DAB substrate kit

Excellent Good 4/5 (Ease of Use)
Abreviews
This kit is streamlined to use at ease. The results is fantastic.
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Abcam user community

Verified customer

投稿 Aug 07 2015

Thank you for your inquiry.

I can suggest to use a strepavidin that is coupled to an enzyme, for example: ab128985 (Streptavidin Peroxidase (Ready-To-Use)) and combine this with a suitable chromogen, for example: ab94665 (DAB substrate kit) <...

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Hello!

In-house we use PBST. Although we do not have any experimental data using other buffers I would imagine that whatever buffer you are using to rinse the slides would be suitable as well.

Please do not hesitate to contact me wi...

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Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the c...

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Thank you for contacting us. You are correct, these are the same product, just different sizes. The appropriate size will depend on how many slides you anticipate needing to stain. If you prepare the substrate/chromogen mixture as suggested in the prot...

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Thank you for contacting us.

We do not currently offer double staining IHC kits. Your staining conditions are challenging as, of the two common HRP chromogens, only DAB is alcohol insoluable. While of the common AP chromagens, only BCIP/NBT ...

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Thank you for contacting us. I would recommend the following protocol for your purposes:

Fixation

Fix the samples either in ice-cold methanol, acetone (1-10 min) or in 3-4% paraformaldehyde in PBS pH 7.4 for 15 min at room temperatu...

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Thank you for contacting us. I'm glad to hear you have been getting good staining with ab469. In order to help you obtain more intense staining would you mind filling in the form which I have attached to this email. It will allow me to more fully unde...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"