製品の概要

  • 製品名Anti-DAAM1 antibody
    DAAM1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DAAM1
  • アプリケーション適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide from the N terminal region of human DAAM1.

  • ポジティブ・コントロール
    • WB: 293 cell lysate IHC-P: human brain tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
IHC-P 1/10 - 1/50.
WB 1/50 - 1/100. Detects a band of approximately 130 kDa (predicted molecular weight: 123 kDa).

ターゲット情報

  • 関連性DAAM1 (Dishevelled associated activator of morphogenesis 1) is a diaphanous-related formin which binds to dishevelled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex assembly. It is an essential component of noncanonical Wnt signaling. DAAM1 also communicates with Rho GTPases, CIP4 and Src in the regulation of the signalling pathways that co-ordinate the dynamics of the actin filament system. DAAM1 may be a coordinator of endocytosis and cytoskeletal remodeling.
  • 細胞内局在Cytoplasmic. Note: Perinuclear.
  • 参照データベース
  • 別名
    • DAAM 1 antibody
    • Dishevelled associated activator of morphogenesis 1 antibody
    • FLJ41657 antibody
    • KIAA0666 antibody

Anti-DAAM1 antibody 画像

  • All lanes : Anti-DAAM1 antibody (ab71327) at 1/50 dilution

    Lane 1 : 293 cell lysate, nontransfected
    Lane 2 : 293 cell lysate, transfected with the DAAM1 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 123 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
  • ab71327, at a 1/10 dilution, staining DAAM1 in formalin fixed and paraffin embedded human brain tissue by Immunohistochemistry. A peroxidase conjugated secondary antibody was then used, followed by DAB staining.

Anti-DAAM1 antibody (ab71327) 使用論文

This product has been referenced in:
  • Rydell-Törmänen K  et al. Aberrant nonfibrotic parenchyma in idiopathic pulmonary fibrosis is correlated with decreased ß-catenin inhibition and increased Wnt5a/b interaction. Physiol Rep 4:N/A (2016). IHC-P ; Human . Read more (PubMed: 26997628) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"