The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 for 10 min at RT. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
WB: Use at a concentration of 1 µg/ml for 2hours. Predicted molecular weight: 23 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them (PubMed:8356058, PubMed:7512369). Regulates reorganization of the actin cytoskeleton mediated by Rho family members (PubMed:8262133).
Lichtenfels R et al. Comparative expression profiling of distinct T cell subsets undergoing oxidative stress. PLoS One7:e41345 (2012).
Read more (PubMed: 22911781) »
Weitzel LR et al. Discovery and verification of protein differences between Er positive/Her2/neu negative breast tumor tissue and matched adjacent normal breast tissue. Breast Cancer Res Treat124:297-305 (2010).
Read more (PubMed: 20087651) »