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This clone has been derived from hybridization of Sp 2/0 myeloma cells with spleen cells of Balb/c mice immunized with homogenized fibrin clot.
Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.
Our Abpromise guarantee covers the use of ab24474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Predicted molecular weight: 1 kDa.|
|Sandwich ELISA||Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [DD1] to D-Dimer (ab10050). Can be used as Detection antibody. Recommended for D-Dimer detection in Human Plasma. Plasma must be diluted with 10 mM Tris/HCl buffer, pH 7.5, containing 1 M NaCl and 0.1 % Tween 20. To avoid non-specific binding, the final NaCl concentration in plasma samples must be 0.5 M or more. Less than 10 ng/ml of D-Dimer can be detected by DD1-DD6 assay using ordinary ELISA approach with HRP-labeled DD6 MAb|