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This clone has been derived from hybridization of Sp 2/0 myeloma cells with spleen cells of Balb/c mice immunized with homogenized fibrin clot.
Our Abpromise guarantee covers the use of ab10053 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent dilution.|
|Sandwich ELISA||Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [DD4] to D-Dimer (HRP) (ab10054). Can be used as Capture antibody. Recommended for D-Dimer detection in Human Plasma. Plasma must be diluted with 10 mM Tris/HCl buffer, pH 7.5, containing 1 M NaCl and 0.1 % Tween 20. To avoid non-specific binding, the final NaCl concentration in plasma samples must be 0.5 M or more. WB: Use at an assay dependent dilution.|
ab10053 has not yet been referenced specifically in any publications.