CytoPainter F-actin Staining Kit - Orange Fluorescence (ab112126)

製品の概要

  • 製品名CytoPainter F-actin Staining Kit - Orange Fluorescence
    F-actin キット 製品一覧
  • サンプルの種類
    Tissue, Adherent cells, Suspension cells
  • アッセイタイプCell-based
  • 製品の概要

    Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.

    ab112126 is designed to stain F-actins in fixed cells with orange fluorescence. ab112126 uses an orange fluorescent phalloidin conjugate that is selectively bound to F-actins. The orange fluorescent phalloidin conjugate has Ex/Em = 550/575 nm. The conjugate also has good photostability for fluorescence imaging. It is a high-affinity probe for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. ab112126 provides all the essential components with an optimized labeling protocol.

  • 特記事項

    ab112126 should be stored dessicated.

  • アプリケーション適用あり: FM, ICC/IFmore details

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab112126 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    FM Use at an assay dependent concentration.
    ICC/IF Use at an assay dependent concentration.

    CytoPainter F-actin Staining Kit - Orange Fluorescence 画像

    • ab112126, staining CPA cells fixed with formaldehyde in a black 96-well plate.
      Left panel: The cells were labeled with Orange Fluorescent Phalloidin Conjugate for 30 minutes only.
      Right panel: The cells were treated with phalloidin for 10 minutes, then stained with Orange Fluorescent Phalloidin Conjugate for 30 minutes.

    プロトコール

    CytoPainter F-actin Staining Kit - Orange Fluorescence (ab112126) 使用論文

    ab112126 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.

    The CytoPainter kits are compatible with secondary antibodies and nuclear dyes for multicolor staining or GFP-transfected cells, for example. The lab has confirmed that these kits can be used with other antibodies...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"