CytoPainter F-actin Staining Kit - Green Fluorescence (ab112125)

製品の概要

  • 製品名CytoPainter F-actin Staining Kit - Green Fluorescence
    F-actin キット 製品一覧
  • サンプルの種類
    Tissue, Adherent cells, Suspension cells
  • アッセイタイプCell-based (qualitative)
  • 製品の概要

    Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.

    ab112125 is designed to stain F-actins in fixed cells with green fluorescence. The kit uses a green fluorescent phalloidin conjugate that is selectively bound to F-actins. This green fluorescent phalloidin conjugate is a high-affinity probe for F-actins. When used at nanomolar concentrations, phallotoxins are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.

    ab112125 provides all the essential components with an optimized staining protocol, which is robust requiring minimal hands-on time. It is an excellent tool for preserving the fluorescent images of particular cells. The phalloidin conjugate has spectral properties similar to those of FITC (Ex/Em = 500/520 nm). It can also be used for fluorescence microscope demonstrations using FITC filter (Ex/Em = 490/525 nm).

  • 特記事項

    ab112125 should be stored dessicated.

  • アプリケーション適用あり: FM, ICC/IFmore details

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab112125 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    FM Use at an assay dependent concentration.
    ICC/IF Use at an assay dependent concentration.

    CytoPainter F-actin Staining Kit - Green Fluorescence 画像

    • Left Panel: Cells labeled with 1X Green Fluorescent Phalloidin Conjugate for 30 minutes only.
      Right Panel: Cells treated with phalloidin for 10 minutes, then stained with Green Fluorescent Phalloidin Conjugate for 30 minutes.

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    CytoPainter F-actin Staining Kit - Green Fluorescence (ab112125) 使用論文

    ab112125 has not yet been referenced specifically in any publications.

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    Abreviews
    The kit is easy to use. This is an advantage, though the fact that it comes with a buffer to use for the fixation protocol does not allow for easy versatility. Is a bit dis courageous to try using it adding other fluoroscopes and knowing which buffer suits the other fluorophores best.
    For STED purposes, it could be used, but we found a bit higher the background noise. This might be as well the buffer, we did not test it further.
    We used not very high STED power and work, but not being better than other dyes we are used to employ for actin staining.
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    Abcam user community

    Verified customer

    投稿 Mar 03 2015

    Thank you for your protocol information.



    Using methanol to dehydrate may be causing damage to the actin stucture. Please consider using the following ethanol dehydration protocol.



    Fixation: 4% PFA in PBS overni...

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    Could you please provide your fixation and embedding protocols? As fas as I know, I have not come across any protocols that required methanol dehydration steps. Hopefully with this information, I will be able to help troubleshoot your experiment.

    Thank you for your enquiry.



    It is best to prepare fresh solution, so if you do not need to use 10ml in one experiment, then you could start with making less working solution (eg 5ul conjugate in 5mL buffer). Please avoid aliquating...

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    Thank you for your enquiry.



    Here are the answers to your questions.



    1) Antigen retrieval will be required if you have used PFA or formalin to fix your tissue. I would suggest trying a section that has been retr...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"