The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報ELISA: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution (CMV infected cells).
IHC-Fr: Use at an assay dependent dilution (CMV infected cells).
Neut: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
関連性Human cytomegalovirus (HCMV) glycoprotein B (gB) is an abundant virion envelope protein that has been shown to be essential for the infectivity of HCMV. HCMV gB is also one of the most immunogenic virus-encoded proteins, and a significant fraction of virus neutralizing antibodies are directed at gB.
CMV glycoprotein B antibody
Glycoprotein B antibody
HHV5 glycoprotein B antibody
Anti-Cytomegalovirus Glycoprotein B antibody [HCMV37] 画像
Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus Glycoprotein B antibody [HCMV37] (ab54023)Image from Haspot F et al., PLoS One. 2012;7(4):e34795. Epub 2012 Apr 9. Fig 2.; doi:10.1371/journal.pone.0034795; April 9, 2012, PLoS ONE 7(4): e34795.
ab54023, staining Cytomegalovirus Glycoprotein B in monocyte-derived dendritic cells infected with Human cytomegalovirus (VHL/E strain), cultured for 3 hours (left panels) and 24 hours (right panels), by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with paraformaldehyde and permeabilized with 0.2% Triton X-100 in PBS for 10 minutes at room temperature. Samples were incubated with primary antibody and an AlexaFluor®568-conjugated goat anti-mouse monoclonal IgG was used as the secondary antibody.
Anti-Cytomegalovirus Glycoprotein B antibody [HCMV37] (ab54023) 使用論文
This product has been referenced in:
Kirchmeier M et al. Enveloped virus-like particle expression of human cytomegalovirus glycoprotein B antigen induces antibodies with potent and broad neutralizing activity. Clin Vaccine Immunol21:174-80 (2014).
Read more (PubMed: 24334684) »
Haspot F et al. Human cytomegalovirus entry into dendritic cells occurs via a macropinocytosis-like pathway in a pH-independent and cholesterol-dependent manner. PLoS One7:e34795 (2012).
Read more (PubMed: 22496863) »