Anti-Cytokeratin 抗体 [MNF116], prediluted (ab82612)


  • 製品名Anti-Cytokeratin antibody [MNF116], prediluted
    Cytokeratin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MNF116] to Cytokeratin, prediluted
  • 特異性This antibody recognizes the keratin polypeptide of 45, 46, and 56.5 kD. It shows a broad pattern of reactivity with human epithelial tissues from simple glandular epithelia to stratified squamous epithelia, like epidermis, mammary gland ducts, and tracheal epithelium.
  • アプリケーション適用あり: Flow Cyt, IHC-P, IHC-Fr, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A crude extract of splenic cells from Nude Mouse engrafted with MCF-7 cells.

  • ポジティブ・コントロール
    • Skin.


  • 製品の状態Prediluted
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, Stabilizing agent, pH 7.5
  • Concentration information loading...
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名MNF116
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野


Our Abpromise guarantee covers the use of ab82612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration. Fix with acetone.
ICC/IF Use at an assay dependent concentration.


  • 機能May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1).
  • 組織特異性The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.
  • 関連疾患Defects in KRT1 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
    Defects in KRT1 are the cause of ichthyosis hystrix Curth-Macklin type (IHCM) [MIM:146590]. IHCM is a genodermatosis with severe verrucous hyperkeratosis. Affected individuals manifest congenital verrucous black scale on the scalp, neck, and limbs with truncal erythema, palmoplantar keratoderma and keratoses on the lips, ears, nipples and buttocks.
    Defects in KRT1 are a cause of palmoplantar keratoderma non-epidermolytic (NEPPK) [MIM:600962]. NEPKK is a dermatological disorder characterized by focal palmoplantar keratoderma with oral, genital, and follicular lesions.
    Defects in KRT1 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
    Defects in KRT1 are the cause of palmoplantar keratoderma striate type 3 (SPPK3) [MIM:607654]; also known as keratosis palmoplantaris striata III. SPPK3 is a dermatological disorder affecting palm and sole skin where stratum corneum and epidermal layers are thickened. There is no involvement of non-palmoplantar skin, and both hair and nails are normal.
  • 配列類似性Belongs to the intermediate filament family.
  • 翻訳後修飾Undergoes deimination of some arginine residues (citrullination).
  • 細胞内局在Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 67 kDa cytokeratin antibody
    • CK-1 antibody
    • CK1 antibody
    • Cytokeratin 1 antibody
    • Cytokeratin 19 antibody
    • Cytokeratin 8 antibody
    • Cytokeratin-1 antibody
    • EHK antibody
    • EHK1 antibody
    • EPPK antibody
    • Hair alpha protein antibody
    • K1 antibody
    • K2C1_HUMAN antibody
    • Keratin 1 antibody
    • Keratin 19 antibody
    • Keratin 8 antibody
    • Keratin antibody
    • Keratin-1 antibody
    • Krt1 antibody
    • KRT19 antibody
    • KRT1A antibody
    • KRT8 antibody
    • KRTA antibody
    • NEPPK antibody
    • type II cytoskeletal 1 antibody
    • Type-II keratin Kb1 antibody
    see all

Anti-Cytokeratin antibody [MNF116], prediluted 画像

  • ab82612 staining Cytokeratin in human tonsil tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

  • ICC/IF image of ab82612 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82612, neat) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing A431 cells stained with ab82612 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82612, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Cytokeratin antibody [MNF116], prediluted (ab82612) 使用論文

ab82612 has not yet been referenced specifically in any publications.

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La concentration du ab82612 est bien de 1 à 3 µg/ml. Cet anticorps est prédilué et prêt à être utilisé. La taille unitaire est de 6 ml.

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L'anticorps prédilué ab82612 (Anti-Cytokeratin [MNF116]) a une concentration entre 1 et 3 µg/ml.
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