Anti-Cytokeratin 8 (phospho S431) 抗体 [EP1630] (ab109452)


  • 製品名Anti-Cytokeratin 8 (phospho S431) antibody [EP1630]
    Cytokeratin 8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1630] to Cytokeratin 8 (phospho S431)
  • 特異性ab109452 only detects Cytokeratin 8 phosphorylated at Serine 431
  • アプリケーション適用あり: WB, IP, IHC-P, ICCmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues surrounding Serine 431 of Human Cytokeratin 8.

  • ポジティブ・コントロール
    • HeLa lysates, treated with FBS and Calyculin; Human ductal breast carcinoma and Human stomach adenocarcinoma tissues.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab109452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 54 kDa).
IP 1/50.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
    • 組織特異性Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
    • 関連疾患Cirrhosis
    • 配列類似性Belongs to the intermediate filament family.
    • 翻訳後修飾Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
      O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
      O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
    • 細胞内局在Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CARD2 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK8 antibody
      • CYK8 antibody
      • CYKER antibody
      • Cytokeratin endo A antibody
      • Cytokeratin-8 antibody
      • DreK8 antibody
      • EndoA antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 8 antibody
      • Keratin type II cytoskeletal 8 antibody
      • Keratin, type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • Krt 2.8 antibody
      • KRT8 antibody
      • MGC118110 antibody
      • MGC174782 antibody
      • MGC53564 antibody
      • MGC85764 antibody
      • sb:cb186 antibody
      • Type-II keratin Kb8 antibody
      see all

    Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] 画像

    • All lanes : Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452) at 1/10000 dilution

      Lane 1 : HeLa lysates, untreated
      Lane 2 : HeLa lysates, treated with FBS and Calyculin A

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 54 kDa
      Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    • ab109452 at 1/100 dilution staining Cytokeratin 8 in Human ductal breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
    • ab109452 at 1/100 dilution staining Cytokeratin 8 in Human stomach adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452) 使用論文

    This product has been referenced in:
    • Kuga T  et al. A novel mechanism of keratin cytoskeleton organization through casein kinase Ia and FAM83H in colorectal cancer. J Cell Sci 126:4721-31 (2013). Saccharomyces cerevisiae . Read more (PubMed: 23902688) »

    See 1 Publication for this product

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