製品の概要

  • 製品名
    Anti-Cytokeratin 8 (phospho S431) antibody
    Cytokeratin 8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cytokeratin 8 (phospho S431)
  • 特異性
    Detects endogenous levels of total Cytokeratin 8 only when phosphorylated at serine 431.
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic phosphopeptide (Human) from around the phosphorylation site of serine 431 (LTSPPG)

  • ポジティブ・コントロール
    • Human colon carcinoma tissue and 293 cells treated with EGF (200ng/ml, 30mins).
  • 特記事項
    Phosphorylation sites: Human: Ser431; Mouse: Ser436; Rat: Ser431

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/40000.

ターゲット情報

  • 機能
    Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • 組織特異性
    Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
  • 関連疾患
    Cirrhosis
  • 配列類似性
    Belongs to the intermediate filament family.
  • 翻訳後修飾
    Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
    O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • 細胞内局在
    Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CARD2 antibody
    • CK 8 antibody
    • CK-8 antibody
    • CK8 antibody
    • CYK8 antibody
    • CYKER antibody
    • Cytokeratin endo A antibody
    • Cytokeratin-8 antibody
    • DreK8 antibody
    • EndoA antibody
    • K2C8 antibody
    • K2C8_HUMAN antibody
    • K8 antibody
    • Keratin 8 antibody
    • Keratin type II cytoskeletal 8 antibody
    • Keratin, type II cytoskeletal 8 antibody
    • Keratin-8 antibody
    • KO antibody
    • Krt 2.8 antibody
    • KRT8 antibody
    • MGC118110 antibody
    • MGC174782 antibody
    • MGC53564 antibody
    • MGC85764 antibody
    • sb:cb186 antibody
    • Type-II keratin Kb8 antibody
    see all

Anti-Cytokeratin 8 (phospho S431) antibody 画像

  • Immunohistochemical analysis of paraffin embedded human colon carcinoma tissue stained with ab59434 at 1/50 dilution; in the presence or absence of the phosphopeptide.
  • All lanes : Anti-Cytokeratin 8 (phospho S431) antibody (ab59434) at 1/500 dilution

    Lane 1 : 293 cell extract treated with EGF (200ng/ml, 30 mins)
    Lane 2 : 293 cell extract treated with EGF (200ng/ml, 30 mins) with phosphopeptide


    Predicted band size : 54 kDa
    Observed band size : 54 kDa

Anti-Cytokeratin 8 (phospho S431) antibody (ab59434) 使用論文

This product has been referenced in:
  • Khapare N  et al. Plakophilin3 Loss Leads to an Increase in PRL3 Levels Promoting K8 Dephosphorylation, Which Is Required for Transformation and Metastasis. PLoS One 7:e38561 (2012). WB ; Human . Read more (PubMed: 22701666) »
  • Alam H  et al. Loss of Keratin 8 Phosphorylation Leads to Increased Tumor Progression and Correlates with Clinico-Pathological Parameters of OSCC Patients. PLoS One 6:e27767 (2011). WB, IHC-P ; Human . Read more (PubMed: 22114688) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (pancreatic cancer cell line)
Loading amount
25 µg
Specification
pancreatic cancer cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
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Verified customer

投稿 Oct 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (lung cancer cell lines H522 and H1792)
Loading amount
25 µg
Specification
lung cancer cell lines H522 and H1792
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Oct 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (endometrial adenocarcinoma)
Specification
endometrial adenocarcinoma
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Oct 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreatic tumor)
Specification
pancreatic tumor
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Oct 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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