製品の概要

  • 製品名Anti-Cytokeratin 8 antibody [TS1] (Biotin)
    Cytokeratin 8 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [TS1] to Cytokeratin 8 (Biotin)
  • 標識Biotin
  • 特異性Highly specific to Cytokeratin 8; shows no cross-reactivity with other keratins. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: IP, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Keratin preparation from a Human carcinoma

  • エピトープaa 343-357
  • ポジティブ・コントロール
    • IP: MCF-7 or A431 cells. IHC-P: Human skin; Human colon, lung or breast carcinoma tissue.
  • 特記事項Stable for 24 months when stored at 4°C.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP
IHC-P
  • 追加情報IHC-P: Use at a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol, by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    IP: Use at 2µg/mg of lysate. Use protein G.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
    • 組織特異性Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
    • 関連疾患Cirrhosis
    • 配列類似性Belongs to the intermediate filament family.
    • 翻訳後修飾Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
      O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
      O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
    • 細胞内局在Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CARD2 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK8 antibody
      • CYK8 antibody
      • CYKER antibody
      • Cytokeratin endo A antibody
      • Cytokeratin-8 antibody
      • DreK8 antibody
      • EndoA antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 8 antibody
      • Keratin type II cytoskeletal 8 antibody
      • Keratin, type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • Krt 2.8 antibody
      • KRT8 antibody
      • MGC118110 antibody
      • MGC174782 antibody
      • MGC53564 antibody
      • MGC85764 antibody
      • sb:cb186 antibody
      • Type-II keratin Kb8 antibody
      see all

    Anti-Cytokeratin 8 antibody [TS1] (Biotin) (ab79471) 使用論文

    This product has been referenced in:
    • Sundström BE  et al. Diversity in immunoreactivity of tumor-derived cytokeratin monoclonal antibodies. J Histochem Cytochem 37:1845-54 (1989). IHC-P ; Human . Read more (PubMed: 2479674) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"