製品の概要

  • 製品名Anti-Cytokeratin 8 antibody [TS1]
    Cytokeratin 8 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [TS1] to Cytokeratin 8
  • 特異性ab49778 recognises cytokeratin 8 intermediate filament.
  • アプリケーション適用あり: IHC-Fr, IHC-P, ICC/IFmore details
    適用なし: WB
  • 種交差性
    交差種: Human
  • 免疫原

    Purified tumour-derived cytokeratins.

  • ポジティブ・コントロール
    • IHC-P: Human lung tissue. ICC/IF: HeLa cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab49778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/100 - 1/200.

Acetone fixation recommended.

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
    • 組織特異性Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
    • 関連疾患Cirrhosis
    • 配列類似性Belongs to the intermediate filament family.
    • 翻訳後修飾Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
      O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
      O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
    • 細胞内局在Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CARD2 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK8 antibody
      • CYK8 antibody
      • CYKER antibody
      • Cytokeratin endo A antibody
      • Cytokeratin-8 antibody
      • DreK8 antibody
      • EndoA antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 8 antibody
      • Keratin type II cytoskeletal 8 antibody
      • Keratin, type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • Krt 2.8 antibody
      • KRT8 antibody
      • MGC118110 antibody
      • MGC174782 antibody
      • MGC53564 antibody
      • MGC85764 antibody
      • sb:cb186 antibody
      • Type-II keratin Kb8 antibody
      see all

    Anti-Cytokeratin 8 antibody [TS1] 画像

    • ICC/IF image of ab49778 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49778, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG(H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

    • Immunostaining of human lung tissue with ab49778.

    Anti-Cytokeratin 8 antibody [TS1] (ab49778) 使用論文

    This product has been referenced in:
    • Holm P  et al. Functional mapping and single chain construction of the anti-cytokeratin 8 monoclonal antibody TS1. Mol Immunol 44:1075-84 (2007). Read more (PubMed: 16989900) »
    • Erlandsson A  et al. In vivo clearing of idiotypic antibodies with antiidiotypic antibodies and their derivatives. Mol Immunol 43:599-606 (2006). Read more (PubMed: 15978666) »

    See all 4 Publications for this product

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