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Synthetic non-phosphopeptide derived from human Cytokeratin 8 around the phosphorylation site of serine 431 (L-T-SP-P-G).
Our Abpromise guarantee covers the use of ab59400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).|
|IHC-P||Use at an assay dependent dilution.|
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells, labeling Cytokeratin 8 with ab59400. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of kidney tissue sections labeling Cytokeratin 8 with ab59400 at 1/100 dilution. Tissue sections were fixed with formaldehyde; heat mediated antigen retrieval was performed using a citric acid. 2% BSA was used to block, followed by incubation with ab59400 in TBS/BSA/azide for 2 hours at 21°C. a polyclonal goat anti-rabbit IgG bitin conjugated secondary antibody was used at 1/300 dilution.