製品の概要

  • 製品名
    Anti-Cytokeratin 8 antibody
    Cytokeratin 8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cytokeratin 8
  • アプリケーション
    適用あり: ICC/IF, IHC-Fr, WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human Cytokeratin 8 around the phosphorylation site of serine 431 (L-T-SP-P-G).

  • ポジティブ・コントロール
    • Human colon carcinoma tissue; EGF treated 293 cell extracts

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IHC-P Use at an assay dependent dilution.
ELISA 1/5000.

ターゲット情報

  • 機能
    Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • 組織特異性
    Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
  • 関連疾患
    Cirrhosis
  • 配列類似性
    Belongs to the intermediate filament family.
  • 翻訳後修飾
    Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
    O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • 細胞内局在
    Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CARD2 antibody
    • CK 8 antibody
    • CK-8 antibody
    • CK8 antibody
    • CYK8 antibody
    • CYKER antibody
    • Cytokeratin endo A antibody
    • Cytokeratin-8 antibody
    • DreK8 antibody
    • EndoA antibody
    • K2C8 antibody
    • K2C8_HUMAN antibody
    • K8 antibody
    • Keratin 8 antibody
    • Keratin type II cytoskeletal 8 antibody
    • Keratin, type II cytoskeletal 8 antibody
    • Keratin-8 antibody
    • KO antibody
    • Krt 2.8 antibody
    • KRT8 antibody
    • MGC118110 antibody
    • MGC174782 antibody
    • MGC53564 antibody
    • MGC85764 antibody
    • sb:cb186 antibody
    • Type-II keratin Kb8 antibody
    see all

画像

  • ab59400 staining Cytokeratin 8 in c57b6 mouse prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citric acid. Samples were then blocked using 3% BSA for 1 hour at 25°C and then incubated with ab59400 at a 1/100 dilution for 18 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.

    See Abreview

  • ICC/IF image of ab59400 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59400, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab59400 staining Cytokeratin 8 in Human breast cancer cells xenografted into nude mice by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formalin and subjected to heat-mediated antigen retrieval in citrate buffer (0.1M Sodium Citrate) prior to blocking with 5% serum for 1 hour at 4°C. The primary antibody was diluted 1/200 in 5% goat serum in PBS and incubated with the sample for 12 hours at 4°C. A Biotin-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/1000.

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  • ab59400 staining Cytokeratin 8 in murine mammary gland duct tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 1% BSA for 1 hour at 23°C and then incubated with ab59400 at a 1/250 dilution for 12 hours at 4°C. The secondary used was an Alexa-Fluor 568 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.

    See Abreview

  • Immunohistochemical analysis of paraffin embedded human colon carcinoma tissue using ab59400 at 1/50 dilution, in the presence (right) and absence (left) of immunising peptide. Then, a polymer secondary antibody was used for detection.
  • All lanes : Anti-Cytokeratin 8 antibody (ab59400) at 1/500 dilution

    Lane 1 : EGF treated (200ng/ml, 30mins) 293 cell extracts
    Lane 2 : EGF treated (200ng/ml, 30mins) 293 cell extracts with immunising peptide


    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • Immunocytochemistry/ Immunofluorescence analysis of HeLa cells, labeling Cytokeratin 8 with ab59400. The picture on the right is blocked with the synthesized peptide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of kidney tissue sections labeling Cytokeratin 8 with ab59400 at 1/100 dilution. Tissue sections were fixed with formaldehyde; heat mediated antigen retrieval was performed using a citric acid. 2% BSA was used to block, followed by incubation with ab59400 in TBS/BSA/azide for 2 hours at 21°C. a polyclonal goat anti-rabbit IgG bitin conjugated secondary antibody was used at 1/300 dilution.

    See Abreview

参考文献

This product has been referenced in:
  • Yu L  et al. MFGE8 regulates TGF-ß-induced epithelial mesenchymal transition in endometrial epithelial cells in vitro. Reproduction 152:225-33 (2016). ICC/IF ; Human . Read more (PubMed: 27340235) »
  • Dai YB  et al. Ablation of Liver X receptors a and ß leads to spontaneous peripheral squamous cell lung cancer in mice. Proc Natl Acad Sci U S A 113:7614-9 (2016). Read more (PubMed: 27335465) »

See all 21 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Developing salivary gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Developing salivary gland
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Dec 02 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Sep 30 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (10%)
Sample
Mouse Cell lysate - whole cell (primary hepatoctyes)
Specification
primary hepatoctyes
Blocking step
I-Block(Applied biosystems) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jul 09 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (c57b6 mouse prostate)
Specification
c57b6 mouse prostate
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric Acid
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mammary Gland duct)
Specification
Mammary Gland duct
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Mar 20 2012

Thank you for contacting us.
I am sorry to hear this customer has been experiencing problems with 2 of our antibodies. Would it be possible to obtain more protocol information too so that we can investigate this in more detail?
I look forward...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary gland)
Specification
Mammary gland
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Apr 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human breast cancer cells xenografted in nude mice)
Specification
human breast cancer cells xenografted in nude mice
Fixative
formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.1 M Sodium Citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Megha Rajaram

Verified customer

投稿 Mar 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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