Anti-Cytokeratin 8 抗体 (ab52949)

製品の概要

  • 製品名Anti-Cytokeratin 8 antibody
    Cytokeratin 8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cytokeratin 8
  • アプリケーション適用あり: WB, IP, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    A synthetic peptide corresponding to residues on the N-terminus of human Cytokeratin 8

  • ポジティブ・コントロール
    • HeLa cell lysate, human gastric adenocarcinoma and HeLa cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab52949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IP 1/60.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
    • 組織特異性Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
    • 関連疾患Cirrhosis
    • 配列類似性Belongs to the intermediate filament family.
    • 翻訳後修飾Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
      O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
      O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
    • 細胞内局在Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CARD2 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK8 antibody
      • CYK8 antibody
      • CYKER antibody
      • Cytokeratin endo A antibody
      • Cytokeratin-8 antibody
      • DreK8 antibody
      • EndoA antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 8 antibody
      • Keratin type II cytoskeletal 8 antibody
      • Keratin, type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • Krt 2.8 antibody
      • KRT8 antibody
      • MGC118110 antibody
      • MGC174782 antibody
      • MGC53564 antibody
      • MGC85764 antibody
      • sb:cb186 antibody
      • Type-II keratin Kb8 antibody
      see all

    Anti-Cytokeratin 8 antibody 画像

    • Anti-Cytokeratin 8 antibody (ab52949) at 1/500 dilution + HeLa cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 54 kDa
      Observed band size : 54 kDa
    • ab52949 at 1/100 dilution staining Cytokeratin 8 in human gastric adenocarcinoma by Immunohistochemistry, Paraffin embedded tissue.
    • ab52949 at 1/100 dilution staining Cytokeratin 8 in HeLa cells by Immunofluorescence.

    Anti-Cytokeratin 8 antibody (ab52949) 使用論文

    ab52949 has not yet been referenced specifically in any publications.

    Product Wall

    Les dilutions recommandées pour ab52949 sont :
    Western Blot : 1/500
    IHC-P et ICC/IF : 1/100 - 1/250
    IP : 1/60

    Pour l'anticorps ab51152, la concentration est de 1mg/ml. L'immunogèneayant servi à produire cet...

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    Je vous confirme que l'anticorpsab52949 est dirigé contre la partie N-terminale de la cytokeratine 8 humaine.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"