Anti-Cytokeratin 8 抗体 (ab51152)


  • 製品名Anti-Cytokeratin 8 antibody
    Cytokeratin 8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cytokeratin 8
  • 特異性Keratin 8 (Ab-73) antibody detects endogenous levels of total Keratin 8 protein.
  • アプリケーション適用あり: Indirect ELISA, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic non-phosphopeptide derived from human Keratin 8 around the phosphorylation site of serine 73 (L-L-SP-P-L).

  • ポジティブ・コントロール
    • Human breast carcinoma tissue, HeLa cell extract treated with Anisomycin at 25µg/ml for 30 minutes. IF/ICC: MCF7 cell line.




Our Abpromise guarantee covers the use of ab51152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Indirect ELISA Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 54 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.


  • 機能Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • 組織特異性Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
  • 関連疾患Cirrhosis
  • 配列類似性Belongs to the intermediate filament family.
  • 翻訳後修飾Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
    O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • 細胞内局在Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CARD2 antibody
    • CK 8 antibody
    • CK-8 antibody
    • CK8 antibody
    • CYK8 antibody
    • CYKER antibody
    • Cytokeratin endo A antibody
    • Cytokeratin-8 antibody
    • DreK8 antibody
    • EndoA antibody
    • K2C8 antibody
    • K2C8_HUMAN antibody
    • K8 antibody
    • Keratin 8 antibody
    • Keratin type II cytoskeletal 8 antibody
    • Keratin, type II cytoskeletal 8 antibody
    • Keratin-8 antibody
    • KO antibody
    • Krt 2.8 antibody
    • KRT8 antibody
    • MGC118110 antibody
    • MGC174782 antibody
    • MGC53564 antibody
    • MGC85764 antibody
    • sb:cb186 antibody
    • Type-II keratin Kb8 antibody
    see all

Anti-Cytokeratin 8 antibody 画像

  • ICC/IF image of ab51152 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51152, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • This image shows human breast carcinoma tissue stained with ab51152 at 1/50 dilution. The cells in the right hand image were treated with the immunising peptide, the cells in the left hand image were not.

Anti-Cytokeratin 8 antibody (ab51152) 使用論文

ab51152 has not yet been referenced specifically in any publications.

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La séquence exacte du peptide utilisécomme immunogène est confidentielle.
L'anticorps polyclonalab51152 reconnait spécifiquement les formes phosphorylées et non-phos...

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Les dilutions recommandées pour ab52949 sont :
Western Blot : 1/500
IHC-P et ICC/IF : 1/100 - 1/250
IP : 1/60

Pour l'anticorps ab51152, la concentration est de 1mg/ml. L'immunogèneayant servi à produire cet...

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