製品の概要

  • 製品名
    Anti-Cytokeratin 8 antibody [35bH11]
    Cytokeratin 8 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [35bH11] to Cytokeratin 8
  • アプリケーション
    適用あり: IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
IHC-Fr
  • 追加情報
    IHC: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method.
    Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
    Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to the immunostaining.
  • ターゲット情報

    • 機能
      Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
    • 組織特異性
      Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
    • 関連疾患
      Cirrhosis
    • 配列類似性
      Belongs to the intermediate filament family.
    • 翻訳後修飾
      Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
      O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
      O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
    • 細胞内局在
      Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CARD2 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK8 antibody
      • CYK8 antibody
      • CYKER antibody
      • Cytokeratin endo A antibody
      • Cytokeratin-8 antibody
      • DreK8 antibody
      • EndoA antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 8 antibody
      • Keratin type II cytoskeletal 8 antibody
      • Keratin, type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • Krt 2.8 antibody
      • KRT8 antibody
      • MGC118110 antibody
      • MGC174782 antibody
      • MGC53564 antibody
      • MGC85764 antibody
      • sb:cb186 antibody
      • Type-II keratin Kb8 antibody
      see all

    Anti-Cytokeratin 8 antibody [35bH11] 画像

    • ab758 - immunohistochemistry

      Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 8, using ABC and DAB chromagen.

    • ab758 staining Cytokeratin 8 in formalin-fixed, paraffin-embedded Human colon tissue by Immunohistochemistry. Staining was detected using DAB.

    プロトコール

    Anti-Cytokeratin 8 antibody [35bH11] (ab758) 使用論文

    This product has been referenced in:
    • Berger R  et al. Androgen-induced differentiation and tumorigenicity of human prostate epithelial cells. Cancer Res 64:8867-75 (2004). Read more (PubMed: 15604246) »

    See 1 Publication for this product

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    Thank you for your inquiry.

    For ab758, the immunogen that was used was cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B. We have not validated this antibody in pig. But the human sequence is 85% homologous with the pig ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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