Anti-Cytokeratin 8 + 18 + 19 抗体 [2A4] (ab41825)


  • 製品名Anti-Cytokeratin 8 + 18 + 19 antibody [2A4]
  • 製品の詳細
    Mouse monoclonal [2A4] to Cytokeratin 8 + 18 + 19
  • アプリケーション適用あり: WB, ICC/IF, IHC-P, IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Rat, Human, Pig
  • 免疫原

    Cytokeratins isolated from cultured pig kidney epithelial cells.

  • 特記事項Balb/c spleen cells used to produce hybridoma.

    This antibody recognizes cytokeratins 8, 18 and 19 typical for simple epithelial cells.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 一次抗体 備考This antibody recognizes cytokeratins 8, 18 and 19 typical for simple epithelial cells.
  • ポリ/モノモノクローナル
  • クローン名2A4
  • アイソタイプIgG1
  • 研究分野


Our Abpromise guarantee covers the use of ab41825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab91353 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • 関連性The cytokeratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. Keratin proteins belong to 2 families: acidic (or type I) and basic (or type II). The acidic keratins are coded by genes KRT9 to 19, the basic keratins by genes KRT1 to 8. Cytokeratins 8, 18 and 19 are encoded by the genes KRT8, 18 and 19 respectively. Type I and II keratins are usually coordinately synthesized in preferential pairs so that at least 1 member of each family is expressed in each epithelial cell, in equal proportions. Cytokeratin 8, together with its filament partner cytokeratin 18, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues. KRT8/18 mutations may predispose to liver disease, including cryptogenic cirrhosis, in humans. Unlike its related family members, cytokeratin 19 (the smallest known acidic cytokeratin at 40kDa) is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. It is often expressed in cultured epithelial cells and in some carcinomas.
  • 細胞内局在Cytoplasmic
  • 参照データベース
  • 別名
    • CK18 antibody
    • CK19 antibody
    • CK8 antibody
    • K18 antibody
    • K19 antibody
    • K8 antibody
    • keratin 18 antibody
    • keratin 19 antibody
    • keratin 8 antibody
    • Keratin type I 40kDa antibody
    • Keratin type I cytoskeletal 18 antibody
    • Keratin type I cytoskeletal 19 antibody
    • Keratin type II cytoskeletal 8 antibody
    • KRT18 antibody
    • KRT19 antibody
    • KRT8 antibody
    see all

Anti-Cytokeratin 8 + 18 + 19 antibody [2A4] 画像

  • IHC image of ab41825 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41825, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing MCF7 cells stained with ab41825 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41825, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • ab41825 staining Cytokeratin 8 + 18 + 19 in the MCF-7 epithelial cell line from Human mammary glands by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde, permeabilized with Triton X-100 0.3% in PBS and blocked with 5% serum for 8 hours at 4°C. Samples were incubated with primary antibody (10ug/ml) for 8 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal(10ug/ml) was used as the secondary antibody.

    See Abreview

Anti-Cytokeratin 8 + 18 + 19 antibody [2A4] (ab41825) 使用論文

This product has been referenced in:
  • Navarro-Tableros V  et al. Recellularization of rat liver scaffolds by human liver stem cells. Tissue Eng Part A 21:1929-39 (2015). IHC-Fr ; Rat . Read more (PubMed: 25794768) »
  • Jung SK  et al. Enhancing effects of serum-rich and cytokine-supplemented culture conditions on developing blastocysts and deriving porcine parthenogenetic embryonic stem cells. J Vet Sci 15:519-28 (2014). ICC/IF . Read more (PubMed: 24962410) »

See all 5 Publications for this product

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Leider sind 0,5% BSA das Maximum, dass das Labeling-Kit toleriert. Daher wäre entweder eine IP notwendig, mit der Sie den Antikörper von BSA aufreinigen notwendig, oder aber Sie benutzen ein weitere...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (MCF-7)
Specification MCF-7
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton-X 100
Blocking step Serum as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

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投稿 Aug 24 2012

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While we do not provide explicit expiration dates for our antibodies, we will guaranteethemfor six months from the date of purchase when stored according to our datasheet instructions. For ab41825 and ab55138, we ...

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Expiration date: October 19th, 2012

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