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RabMAb

Anti-Cytokeratin 7 抗体 [EP1620Y] (ab68460)

製品の概要

  • 製品名
    Anti-Cytokeratin 7 antibody [EP1620Y]
    Cytokeratin 7 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1620Y] to Cytokeratin 7
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IPmore details
    適用なし: Flow Cyt or ICC/IF
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human Cytokeratin 7 aa 450-550 (C terminal).

  • ポジティブ・コントロール
    • HeLa cell lysate; HeLa cells; T-47D; SK-BR-3; Human placenta.
  • 特記事項

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    EP1620Y
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab68460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/20000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IP 1/20.

For unpurified use at 1/50. 

  • 追加情報
    Is unsuitable for Flow Cyt or ICC/IF.
  • ターゲット情報

    • 機能
      Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
    • 組織特異性
      Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
    • 配列類似性
      Belongs to the intermediate filament family.
    • 翻訳後修飾
      Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CK 7 antibody
      • CK-7 antibody
      • CK7 antibody
      • Cytokeratin 7 antibody
      • Cytokeratin-7 antibody
      • D15Wsu77e antibody
      • K2C7 antibody
      • K2C7_HUMAN antibody
      • K7 antibody
      • Keratin 7 antibody
      • Keratin 7, type II antibody
      • Keratin type II cytoskeletal 7 antibody
      • Keratin, 55K type II cytoskeletal antibody
      • Keratin, simple epithelial antibody
      • Keratin, simple epithelial type I, K7 antibody
      • Keratin, type II cytoskeletal 7 antibody
      • Keratin-7 antibody
      • Krt2-7 antibody
      • KRT7 antibody
      • MGC11625 antibody
      • MGC129731 antibody
      • MGC3625 antibody
      • Sarcolectin antibody
      • SCL antibody
      • Type II mesothelial keratin K7 antibody
      • Type-II keratin Kb7 antibody
      see all

    画像

    • ab68460 (purified) at 1:20 dilution (0.6µg) immunoprecipitating Cytokeratin 7 in T-47D whole cell lysate.
      Lane 1 (input): T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate 10μg
      Lane 2 (+): ab68460 & T-47D whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68460 in T-47D whole cell lysate
      For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • All lanes : Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460) at 1/20000 dilution (purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg/ml
      Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates at 20 µg/ml
      Lane 3 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg/ml
      Lane 4 : Human placenta lysates at 20 mg/ml

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 51 kDa



      Blocking and diluting buffer : 5% NFDM/TBST

      ~45 kDa bands are supported by the literature and competitor’s products, which may be an isoform.[PMID:16265353]

    • Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460) at 1/20000 dilution (unpurified) + HeLa cell lysate at 10 µg

      Secondary
      HRP conjugated Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 51 kDa
      Observed band size: 51 kDa

    参考文献

    ab68460 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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