Anti-Cytokeratin 7 抗体 [EP1620Y] (ab68460)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1620Y] to Cytokeratin 7
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cytokeratin 7 antibody [EP1620Y]
Cytokeratin 7 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1620Y] to Cytokeratin 7 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Cytokeratin 7 aa 450-550 (C terminal).
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ポジティブ・コントロール
- WB: HeLa, T-47D, SK-BR-3, A549 and placenta cell lysates
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1620Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68460の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/10000 - 1/20000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
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IP |
1/20.
For unpurified use at 1/50. |
特記事項 |
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WB
1/10000 - 1/20000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
IP
1/20. For unpurified use at 1/50. |
ターゲット情報
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機能
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). -
組織特異性
Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
Arg-20 is dimethylated, probably to asymmetric dimethylarginine. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 3855 Human
- Omim: 148059 Human
- SwissProt: P08729 Human
- Unigene: 411501 Human
- Unigene: 670221 Human
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別名
- CK 7 antibody
- CK-7 antibody
- CK7 antibody
see all
画像
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All lanes : Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460) at 1/10000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : KRT7 knockout A549 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 40-55 kDa why is the actual band size different from the predicted?Western blot: Anti-KRT7 antibody [EP1620Y] (ab68460) staining at 1/10000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab68460 was shown to bind specifically to KRT7. A band was observed at 40-55 kDa in wild-type A549 cell lysates with no signal observed at this size in KRT7 knockout cell line. To generate this image, wild-type and KRT7 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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ab68460 (purified) at 1:20 dilution (0.6µg) immunoprecipitating Cytokeratin 7 in T-47D whole cell lysate.
Lane 1 (input): T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate 10μg
Lane 2 (+): ab68460 & T-47D whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68460 in T-47D whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
All lanes : Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460) at 1/20000 dilution (purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg/ml
Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg/ml
Lane 3 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg/ml
Lane 4 : Human placenta tissue lysate at 20 mg/ml
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 51 kDaBlocking and diluting buffer : 5% NFDM/TBST
~45 kDa bands are supported by the literature and competitor’s products, which may be an isoform.[PMID:16265353]
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Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460) at 1/20000 dilution (unpurified) + HeLa cell lysate at 10 µg
Secondary
HRP conjugated Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab68460 は論文での使用が確認できていません。