Anti-Cytokeratin 6 抗体 [Ks6.KA12] (ab18586)

製品の概要

  • 製品名
    Anti-Cytokeratin 6 antibody [Ks6.KA12]
    Cytokeratin 6 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Ks6.KA12] to Cytokeratin 6
  • 特異性
    Cytokeratin 6 antibody [Ks6.KA12](ab18586) represents an excellent marker for non keratinized squamous epithelia and proliferating cells of epidermis (e.g. within psoriatic lesions).
  • アプリケーション
    適用あり: Flow Cyt, ICC, WB, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A cytoskeletal preparation of human callus material was used for immunization.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab18586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
IHC-P 1/10. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 組織特異性
    Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.
  • 配列類似性
    Belongs to the intermediate filament family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CK 6A antibody
    • CK 6B antibody
    • CK 6C antibody
    • CK 6D antibody
    • CK 6E antibody
    • CK-6C antibody
    • CK-6E antibody
    • Cytokeratin 6a antibody
    • Cytokeratin 6B antibody
    • Cytokeratin 6C antibody
    • Cytokeratin 6D antibody
    • Cytokeratin 6E antibody
    • Cytokeratin-6C antibody
    • Cytokeratin-6E antibody
    • K2C6C_HUMAN antibody
    • K6a keratin antibody
    • K6b keratin antibody
    • K6C antibody
    • K6c keratin antibody
    • K6d keratin antibody
    • K6e keratin antibody
    • Keratin antibody
    • Keratin K6h antibody
    • Keratin type II cytoskeletal 6A antibody
    • Keratin type II cytoskeletal 6B antibody
    • Keratin type II cytoskeletal 6C antibody
    • Keratin type II cytoskeletal 6D antibody
    • Keratin type II cytoskeletal 6E antibody
    • Keratin-6C antibody
    • KRT6A antibody
    • KRT6B antibody
    • KRT6C antibody
    • KRT6D antibody
    • KRT6E antibody
    • type II cytoskeletal 6C antibody
    • Type-II keratin Kb12 antibody
    see all

画像

  • ab18586 staining Cytokeratin 6 in human breast gland tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with methanol and blocked with 10% serum for 5 minutes. The sample was incubated with primary antibody (1/25) for 1 hour. An HRP-conjugated rabbit polyclonal to mouse IgG was used undiluted as secondary antibody. Ultravision ONE detection system has been used.

    See Abreview

  • ab18586 staining Cytokeratin 6 in breast organoids derived from normal human breast tissue by Immunocytochemistry.
    Cells were fixed in methanol and blocked using 10% serum for 5 minutes at 25°C. Samples were then incubated with ab18586 at a 1/25 for 1 hour at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal.

    See Abreview

  • Overlay histogram showing A431 cells stained with ab18586 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18586, 2µg/1x106 cells ) for 30 min at 22°C. The secondary antibody used was a goat anti-mous DyLight® 488 (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.

参考文献

This product has been referenced in:
  • Holt BM  et al. Pig dorsum model for examining impaired wound healing at the skin-implant interface of percutaneous devices. J Mater Sci Mater Med 24:2181-93 (2013). Read more (PubMed: 23832453) »

See 1 Publication for this product

レビューと Q&A

Thank you for your enquiry. I have contacted the laboratory that provides the mouse monoclonal anti-cytokeratin 6 to us, ab18586. I am assuming they have data demonstrating mouse reactivity but I need to confirm this. If they do have data, then our gua...

Read More

Thank you for your call today and for letting us know about the trouble with this antibody. I am sorry for the variability in the results, and I'm hoping that the replacement vial will work as expected.

The free of charge replacement vial wil...

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Epithelial)
Specification
Epithelial
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Mr. Daniel Holod-Betz

Verified customer

投稿 May 25 2012

Thank you for contacting Abcam. The antibody ab18586 recognizes all 6 isoforms of keratin. I have searched our catalogue to find a antibody specific to CK6b, however I have not been able to find one. I would recommend searching www.biocompare.c...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (Primary breast)
Specification
Primary breast
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Rene Villadsen

Verified customer

投稿 Jun 27 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Breast gland)
Specification
Breast gland
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10%
Username

Dr. Rene Villadsen

Verified customer

投稿 Sep 25 2009

Thank you for your enquiry. I have received a reply from the originators of ab18586 and ab25820. The concentration ab18586 is 5mg/ml and the concentration of ab25820 is 2ug/ml. Please don't hesitate to contact us again if you have further questi...

Read More

Thank you for your enquiry. To our knowledge, these antibodies have not yet been tested for cross-reactivity with nectin 1. If we receive future information regarding specificity, we will add such information to the online datasheets. If you have an...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録