製品の概要

  • 製品名Anti-Cytokeratin 5 + 8 antibody [RCK102]
  • 製品の詳細
    Mouse monoclonal [RCK102] to Cytokeratin 5 + 8
  • 特異性This antibody reacts with cytokeratin 5 and cytokeratin 8, recognizing virtually all epithelial tissues and carcinomas.
  • アプリケーション適用あり: ICC/IF, Dot blot, Flow Cyt, IHC-Fr, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Hamster, Dog, Human, Pig
  • 免疫原

    Human lung cancer cell line (MR21).

  • 特記事項


    Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 一次抗体 備考Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
  • ポリ/モノモノクローナル
  • クローン名RCK102
  • ミエローマSp2/0
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
Dot blot 1/100 - 1/1000.
Flow Cyt 1/100 - 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/1000.

ターゲット情報

  • 関連性Cytokeratin 5 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
  • 細胞内局在Cell Membrane and Cytoplasmic
  • 参照データベース
  • 別名
    • CK5 antibody
    • CK8 antibody
    • CYK 8 antibody
    • CYK8 antibody
    • Cytokeratin 5 antibody
    • Cytokeratin 8 antibody
    • K5 antibody
    • K8 antibody
    • Keratin 5 antibody
    • Keratin 8 antibody
    • Keratin type II cytoskeletal 5 antibody
    • Keratin type II cytoskeletal 8 antibody
    • KRT5 antibody
    • KRT8 antibody
    see all

Anti-Cytokeratin 5 + 8 antibody [RCK102] 画像

  • IHC image of ab9005 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9005, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing A431 cells stained with ab9005 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9005, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

  • Immunohistochemistry analysis on frozen sections of human liver hepatocytes labeling Cytokeratin 5 + 8 using ab9005.

  • Immunohistochemistry analysis on frozen sections of human liver hepatocytes and bile ducts labeling Cytokeratin 5 + 8 using ab9005.

  • Immunohistochemistry analysis on frozen sections of human urinary bladder epithelium labeling Cytokeratin 5 + 8 using ab9005.

Anti-Cytokeratin 5 + 8 antibody [RCK102] (ab9005) 使用論文

This product has been referenced in:
  • Sathyanesan M  et al. A molecular characterization of the choroid plexus and stress-induced gene regulation. Transl Psychiatry 2:e139 (2012). IHC-Fr ; Rat . Read more (PubMed: 22781172) »
  • Skibinski G  et al. Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor. Am J Physiol Lung Cell Mol Physiol 293:L69-76 (2007). Read more (PubMed: 17384084) »

See all 5 Publications for this product

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The epithelial monolayer should be fine to you.

The Abpromise guarantee comes with all the tested applications and species mentioned on the datasheet. In that case theproducts are not eligible for Abrev...

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Thank you for contacting us.

The Cytokeratin 5 is about 84% similar between human and rabbit so ab9005 can also cross react. This antibody is also eligible for discount code offer.

At Abcam we have started using ICC/IF (Immunocytoc...

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Thank you for contacting us.
As discussed yesterday I can confirm that these antibodies can be used in ICC/IF.
The ab90102 is most likely to cross react with rabbit protein also. The sequence homology of human Cytokeratin 8 with Rabbit protei...

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There is a very high chance that this antibody will recognise melanocytes. However, being as it has not been specifically tested on melanocytes we can't be certain

Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we offer a replacement or reimbursement. We do not routinely offer free samples. We also offer a USD20 Amazon gift voucher to researchers who feedback information a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"