製品の概要

  • 製品名Anti-Cytokeratin 5 + 18 antibody [C-50]
  • 製品の詳細
    Mouse monoclonal [C-50] to Cytokeratin 5 + 18
  • 特異性This antibody recognises the 58 kDa Cytokeratin 5 and the 45 kDa Cytokeratin 18.
  • アプリケーション適用あり: ICC/IF, Flow Cyt, IHC-P, IP, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Hamster, Cow, Dog, Human, Pig
    非交差種: Chicken, Xenopus laevis
  • 免疫原

    Tissue, cells or virus corresponding to Human Cytokeratin 5 + 18.

  • 特記事項This antibody is strongly positive on simple and stratified epithelia.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度>95% by SDS-PAGE
  • 特記事項(精製)Purified from ascites by protein A-affinity chromatography.
  • ポリ/モノモノクローナル
  • クローン名C-50
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use 1µg for 106 cells.
IHC-P Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

ターゲット情報

Anti-Cytokeratin 5 + 18 antibody [C-50] 画像

  • ICC/IF image of ab2535 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2535, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing A431 cells stained with ab2535 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2535, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Cytokeratin 5 + 18 antibody [C-50] (ab2535) 使用論文

This product has been referenced in:
  • Bártek J  et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol 164:215-24 (1991). Read more (PubMed: 1716305) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Dog Cell lysate - whole cell (Dog Lens Epithelial (DLE) cells (primary culture))
Specification Dog Lens Epithelial (DLE) cells (primary culture)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5%
Username

Miss. Amanda Agler

Verified customer

投稿 Jan 31 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"