Anti-Cytokeratin 5 + 14 抗体 [LH8] (ab20203)
Key features and details
- Mouse monoclonal [LH8] to Cytokeratin 5 + 14
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Human
- Isotype: IgM
製品の概要
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製品名
Anti-Cytokeratin 5 + 14 antibody [LH8]
Cytokeratin 5 + 14 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [LH8] to Cytokeratin 5 + 14 -
由来種
Mouse -
特異性
Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen. -
アプリケーション
適用あり: IHC-Fr, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
The 50,000 Mr band (including K14 )cut from an SDS-polyacrylamide gel and Triton X-100 extracted cytoskeleton pellet from cultured human foreskin keratinocytes.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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精製度
Protein A/G purified -
ポリ/モノ
モノクローナル -
クローン名
LH8 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgM -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab20203の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. PubMed: 24705787
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特記事項 |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. PubMed: 24705787 |
ターゲット情報
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関連性
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (i.e. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. -
細胞内局在
Cytoplasmic; Basal layer -
参照データベース
- Entrez Gene: 3852 Human
- Entrez Gene: 3861 Human
- Omim: 148040 Human
- Omim: 148066 Human
- SwissProt: P02533 Human
- SwissProt: P13647 Human
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別名
- CK 14 antibody
- CK 5 antibody
- DDD antibody
see all
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab20203 は 2 報の論文で使用されています。
- Moser B et al. Expression of RAGE and HMGB1 in thymic epithelial tumors, thymic hyperplasia and regular thymic morphology. PLoS One 9:e94118 (2014). IHC-P ; Human . PubMed: 24705787
- Prince ME et al. Identification of a subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma. Proc Natl Acad Sci U S A 104:973-8 (2007). IHC-Fr ; Human . PubMed: 17210912