製品の概要

  • 製品名Anti-Cytokeratin 20 antibody [Ks20.8], prediluted
    Cytokeratin 20 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Ks20.8] to Cytokeratin 20, prediluted
  • 特異性Cytokeratin 20 is a 46 kDa intermediate filament protein whose expression is restricted primarily to gastric and intestinal epithelium, urothelium, and Merkel cells. This antibody is highly specific to cytokeratin 20 and shows no cross-reactivity with other IFPs. Cytokeratin 20 is a unique type I keratin that is expressed in adenocarcinomas of the colon stomach, pancreas and bile system. It is also expressed in mucinous ovarian tumors, transitional cell carcinomas of the urinary tract, and Merkel cell carcinomas. This antibody is essentially non-reactive in squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas. This antibody is often used in conjunction with CK7 and other antibodies to distinguishing colon carcinomas (CK20+), from ovarian, pulmonary, and breast carcinomas.
  • アプリケーション適用あり: IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Cytoskeletal preparation from microdissected villi of human duodenal mucosa.

  • ポジティブ・コントロール
    • Colon carcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
  • 組織特異性Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
  • 配列類似性Belongs to the intermediate filament family.
  • 発生段階First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.
  • 翻訳後修飾Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
    Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD20 antibody
    • CK 20 antibody
    • CK-20 antibody
    • CK20 antibody
    • Cytokeratin-20 antibody
    • Cytokeratin20 antibody
    • K1C20_HUMAN antibody
    • K20 antibody
    • KA20 antibody
    • Keratin 20 antibody
    • keratin 20, type I antibody
    • keratin 21, rat, homolog of antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 20 antibody
    • Keratin-20 antibody
    • Keratin20 antibody
    • KRT 20 antibody
    • KRT 21 antibody
    • KRT20 antibody
    • KRT21 antibody
    • MGC35423 antibody
    • OTTHUMP00000164518 antibody
    • Protein IT antibody
    • type I cytoskeletal 20 antibody
    see all

Anti-Cytokeratin 20 antibody [Ks20.8], prediluted (ab962) 使用論文

This product has been referenced in:
  • Volkmer JP  et al. Three differentiation states risk-stratify bladder cancer into distinct subtypes. Proc Natl Acad Sci U S A 109:2078-83 (2012). IHC-Fr ; Human . Read more (PubMed: 22308455) »
  • Fang DD  et al. Expansion of CD133(+) colon cancer cultures retaining stem cell properties to enable cancer stem cell target discovery. Br J Cancer 102:1265-75 (2010). Flow Cyt ; Human . Read more (PubMed: 20332776) »

See all 8 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"