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RabMAb

Anti-Cytokeratin 20 抗体 [EPR1621(2)] (ab109111)

製品の概要

  • 製品名
    Anti-Cytokeratin 20 antibody [EPR1621(2)]
    Cytokeratin 20 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1621(2)] to Cytokeratin 20
  • アプリケーション
    適用あり: Flow Cyt, ICC/IF, WB, IPmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human Cytokeratin 20 (P35900).

  • ポジティブ・コントロール
    • WB: SW480, Mouse small intestine and Rat small intestine lysates ICC/IF: SW480 cells
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
WB 1/10000 - 1/50000. Detects a band of approximately 46 kDa (predicted molecular weight: 48 kDa).
IP 1/10 - 1/100.
  • 追加情報
    Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能
      Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
    • 組織特異性
      Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
    • 配列類似性
      Belongs to the intermediate filament family.
    • 発生段階
      First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.
    • 翻訳後修飾
      Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
      Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD20 antibody
      • CK 20 antibody
      • CK-20 antibody
      • CK20 antibody
      • Cytokeratin-20 antibody
      • Cytokeratin20 antibody
      • K1C20_HUMAN antibody
      • K20 antibody
      • KA20 antibody
      • Keratin 20 antibody
      • keratin 20, type I antibody
      • keratin 21, rat, homolog of antibody
      • Keratin antibody
      • Keratin type I cytoskeletal 20 antibody
      • Keratin-20 antibody
      • Keratin20 antibody
      • KRT 20 antibody
      • KRT 21 antibody
      • KRT20 antibody
      • KRT21 antibody
      • MGC35423 antibody
      • OTTHUMP00000164518 antibody
      • Protein IT antibody
      • type I cytoskeletal 20 antibody
      see all

    画像

    • Flow Cytometry analysis of SW480 (human colorectal adenocarcinoma ) cells labeling Cytokeratin 20 with unpurified ab109111 at 1:20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • All lanes : Anti-Cytokeratin 20 antibody [EPR1621(2)] (ab109111) at 1/10000 dilution

      Lane 1 : SW480 lysate
      Lane 2 : Mouse small intestine lysate
      Lane 3 : Rat small intestine lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 48 kDa
      Observed band size : 46 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of Cytokeratin 20 in SW480 cells, using ab109111 at a 1/50 dilution.

    参考文献

    ab109111 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample
    Rat Tissue sections (Bladder)
    Specification
    Bladder
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Jan 13 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample
    Marmoset (common) Tissue sections (Small intestine)
    Specification
    Small intestine
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Jan 13 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample
    Goat Tissue sections (Small intestine)
    Specification
    Small intestine
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Jan 13 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample
    Human Tissue sections (Colon)
    Specification
    Colon
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Jan 13 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample
    Dog Tissue sections (Small intestine)
    Specification
    Small intestine
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Jan 13 2014

    Thank you for your reply.
    I have processed the request to receive a vial of ab76126 as a free of charge replacement for ab10911. Your new order number is *.
    If there is anything else I can help you with, please let me know.

    Thank you for your reply.
    I am sorry that the protocol tips that you received back in February did not prove to be successful and I would be very happy to send you a replacement vial. However, instead of send another vial of ab109111, would you wis...

    Read More

    Thank you for contacting Abcam.
    I just have a few further questions for you, when you increased the primary antibody concentration from 1/10000, which was the primary antibody concentration that you used? I ask as it may be that you need to increa...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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