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Synthetic peptide corresponding to residues in Human Cytokeratin 20 (P35900).
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
A trial size is available to purchase for this antibody.
Our Abpromise guarantee covers the use of ab109111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|ICC/IF||1/50 - 1/100.|
|WB||1/10000 - 1/50000. Detects a band of approximately 46 kDa (predicted molecular weight: 48 kDa).|
|IP||1/10 - 1/100.|
Flow Cytometry analysis of SW480 (human colorectal adenocarcinoma ) cells labeling Cytokeratin 20 with unpurified ab109111 at 1:20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
ab109111 has not yet been referenced specifically in any publications.