Cytokeratin 19 overexpression 293T lysate (whole cell) (ab94060)
製品の概要
-
製品名
Cytokeratin 19 overexpression 293T lysate (whole cell)
Cytokeratin 19 ライゼート 製品一覧 -
特記事項
ab94060 is a 293T cell transfected lysate in which Human Cytokeratin 19 has been transiently over-expressed using a pCMV-Cytokeratin 19 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
-
アプリケーション
適用あり: WBmore details
製品の特性
-
Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
バッファー
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
研究分野
-
背景
Developmental stage: Present in hair follicles at all stages of development. Domain: This keratin differs from all other IF proteins in lacking the C-terminal tail domain. Function: Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Similarity: Belongs to the intermediate filament family. Tissue specificity: Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab94060の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use at an assay dependent dilution.
|
特記事項 |
---|
WB
Use at an assay dependent dilution. |
画像
-
ab94060 at 15µg/lane on an SDS-PAGE gel.
-
All lanes : Anti-Cytokeratin 19 antibody (ab90440) at 1/500 dilution
Lane 1 :Cytokeratin 19 overexpression 293T lysate (whole cell) (ab94060)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab94060 は論文での使用が確認できていません。