製品の概要

  • 製品名
    Anti-Cytokeratin 19 antibody
    Cytokeratin 19 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cytokeratin 19
  • アプリケーション
    適用あり: IHC - Wholemount, WB, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) C terminal (Human).

  • ポジティブ・コントロール
    • Skin.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab15463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC - Wholemount Use at an assay dependent concentration. Used at a dilution of 1/200 for 24 hr on mouse whole mounts of tail skin (see Abreview submitted by Jesus Espada).
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil in citrate buffer for 10 minutes, cool at room temperature for 20 minutes. After blocking, incubate primary antibody 1:100 for 10 minutes at room temperature.

IHC-Fr 1/50.

ターゲット情報

  • 機能
    Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • 組織特異性
    Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
  • 配列類似性
    Belongs to the intermediate filament family.
  • 発生段階
    Present in hair follicles at all stages of development.
  • ドメイン
    This keratin differs from all other IF proteins in lacking the C-terminal tail domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 40 kDa keratin intermediate filament antibody
    • CK 19 antibody
    • CK-19 antibody
    • CK19 antibody
    • Cytokeratin 19 antibody
    • Cytokeratin-19 antibody
    • K19 antibody
    • K1C19_HUMAN antibody
    • K1CS antibody
    • Keratin 19 antibody
    • Keratin type I 40 kD antibody
    • Keratin type I 40kD antibody
    • Keratin type I cytoskeletal 19 antibody
    • Keratin, type I cytoskeletal 19 antibody
    • Keratin, type I, 40 kd antibody
    • Keratin-19 antibody
    • KRT19 antibody
    • MGC15366 antibody
    see all

Anti-Cytokeratin 19 antibody 画像

  • Human skin stained with anti-Cytokeratin 19 antibody (ab15463).
  • All lanes : Anti-Cytokeratin 19 antibody (ab15463) at 1 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 40 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
  • ab15463 staining Cytokeratin 19 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in blocking buffer) for 16 hours at 22°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse developing skin tissue labeling Cytokeratin 19 with ab15463 at 1/500 dilution. The tissue was fixed with formaldehyde; heat-mediated antigen retrieval was performed using a citric acid. Tissue was blocked with 2% BSA for 10 minutes at 21°C. A polyclonal goat anti-rabbit IgG biotin conjugated antibody was used ar 1/300 dilution.  Image shows strong immunopositivity in the cytoplasm of basal cells of the epidermis and a moderate hair follicle cell positivity.

    See Abreview

  • This image shows formalin-fixed mouse skin stained with Cytokeratin 19 antibody (1/50 dilution) after pepsin digestion.
  • ab15463 staining Cytokeratin 19 in the Mouse skin tissue sections by IHC-Fr (Frozen Sections). Tissue samples were fixed with methanol and blocked with 3% Goat serum for 30 minutes at 20°C. The sample was incubated with primary antibody (1/50 in PBS) at 4°C for 14 hours. An HRP-conjugated goat polyclonal to rabbit IgG (1/100) was used as secondary antibody.

    See Abreview

Anti-Cytokeratin 19 antibody (ab15463) 使用論文

This product has been referenced in:
  • He N  et al. Isolation and characterization of hair follicle stem cells from Arbas Cashmere goat. Cytotechnology 68:2579-2588 (2016). Read more (PubMed: 27193423) »
  • Singh SK  et al. Antithetical NFATc1-Sox2 and p53-miR200 signaling networks govern pancreatic cancer cell plasticity. EMBO J 34:517-30 (2015). Read more (PubMed: 25586376) »

See all 21 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate PH 6.0
Permeabilization
No
Specification
pancreas
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 04 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Colon
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Feb 13 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Developing skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Developing skin
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Dec 02 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
BiocareMedical's Rodent Block M as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Mar 05 2013

Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring prod...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Blocking step
PBS + 0.1% Triton X-100, 1% BSA, 10% goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Mr. justin kenkel

Verified customer

投稿 Jul 27 2012

Thank you for taking the time to complete our questionnaire and sending me the publication. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibodies.

The details you have kindly provided will enable us ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mammary Gland duct)
Specification
Mammary Gland duct
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Mar 20 2012

Thank you for contacting us.

I am sorry to hear that the antibody is giving you problems.

As we discussed over the phone, please let me know what staining patternthe MCF7 cells give and we can discuss further how to proceed.
...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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1-10 of 32 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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