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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) C terminal (Human).
Our Abpromise guarantee covers the use of ab15463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC - Wholemount||Use at an assay dependent concentration. Used at a dilution of 1/200 for 24 hr on mouse whole mounts of tail skin (see Abreview submitted by Jesus Espada).|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).|
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Boil in citrate buffer for 10 minutes, cool at room temperature for 20 minutes. After blocking, incubate primary antibody 1:100 for 10 minutes at room temperature.