特記事項（精製）ab75747 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
機能Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
組織特異性Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
関連疾患Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
配列類似性Belongs to the intermediate filament family.
翻訳後修飾Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
ab75747, at a 1/50 dilution, staining Cytokeratin 18 in paraffin embedded human breast carcinoma tissue by Immunohistochemistrytissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunizing peptide.
Western blot - Cytokeratin 18 (phospho S33) antibody (ab75747)
All lanes : Anti-Cytokeratin 18 (phospho S33) antibody (ab75747) at 1/500 dilution
Lane 1 : A431 cell extracts with immunising peptide Lane 2 : A431 cell extracts Lane 3 : EC-304 cell extracts
Predicted band size : 48 kDa Observed band size : 48 kDa