Anti-Cytokeratin 18 (phospho S33) 抗体 (ab75747)

製品の概要

  • 製品名
    Anti-Cytokeratin 18 (phospho S33) antibody
    Cytokeratin 18 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cytokeratin 18 (phospho S33)
  • 特異性
    ab75747 detects endogenous levels of Cytokeratin 18 only when phosphorylated at serine 33.
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic phosphopeptide derived from human Cytokeratin 18 around the phosphorylation site of serine 33 (A-A-SP-V-Y).

  • ポジティブ・コントロール
    • Human breast carcinoma tissue; extracts from A431 cells and EC-304 cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab75747 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 48 kDa.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • 組織特異性
    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • 関連疾患
    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • 配列類似性
    Belongs to the intermediate filament family.
  • 翻訳後修飾
    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • 細胞内局在
    Cytoplasm > perinuclear region.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

画像

  • ab75747, at a 1/50 dilution, staining Cytokeratin 18 in paraffin embedded human breast carcinoma tissue by Immunohistochemistrytissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunizing peptide.
  • All lanes : Anti-Cytokeratin 18 (phospho S33) antibody (ab75747) at 1/500 dilution

    Lane 1 : A431 cell extracts with immunising peptide
    Lane 2 : A431 cell extracts
    Lane 3 : EC-304 cell extracts


    Predicted band size : 48 kDa
    Observed band size : 48 kDa

参考文献

ab75747 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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