製品の概要

  • 製品名Anti-Cytokeratin 18 antibody [RGE53]
    Cytokeratin 18 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [RGE53] to Cytokeratin 18
  • 特異性This antibody reacts exclusively with cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them
  • アプリケーション適用あり: ICC/IF, Dot blot, Flow Cyt, IHC-Fr, WBmore details
  • 種交差性
    交差種: Human, Pig, Zebrafish
    交差が予測される動物種: Mouse, Rat, Rabbit, Chicken, Hamster, Dog
  • 免疫原

    Cytoskeletal preparation of cells

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HeLa and MCF7 whole cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
Dot blot 1/100 - 1/1000.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/100 - 1/200.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

ターゲット情報

  • 機能Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • 組織特異性Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • 関連疾患Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • 配列類似性Belongs to the intermediate filament family.
  • 翻訳後修飾Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • 細胞内局在Cytoplasm > perinuclear region.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Anti-Cytokeratin 18 antibody [RGE53] 画像

  • Immunofluorescence staining images of 9 day old zebrafish embryos.
    ab9217 reacts with with intestinal epithelium and epidermis. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.0
  • All lanes : Anti-Cytokeratin 18 antibody [RGE53] (ab9217) at 5 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 48 kDa


    Exposure time : 30 seconds
  • Immunofluorescence staining images of 9 day old zebrafish embryos.
    ab9217 reacts with intestinal epithelium and epidermis. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.
  • ab9217 at a 1/100 dilution staining Cytokeratin 18 in human adipose tissue-derived stromal cells by Immunocytochemistry/ Immunofluorescence.
  • Immunohistochemistry on frozen section of human kidney epithelium
  • Immunohistochemistry on frozen section of human colon
  • Immunohistochemistry on frozen section of swine liver hepatocytes
  • Overlay histogram showing MCF7 cells stained with ab9217 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9217, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Cytokeratin 18 antibody [RGE53] (ab9217) 使用論文

This product has been referenced in:
  • Montalbano M  et al. Modeling of Hepatocytes Proliferation Isolated from Proximal and Distal Zones from Human Hepatocellular Carcinoma Lesion. PLoS One 11:e0153613 (2016). WB, IHC-P ; Human . Read more (PubMed: 27074018) »
  • Liu J  et al. Cell-to-cell contact induces human adipose tissue-derived stromal cells to differentiate into urothelium-like cells in vitro. Biochem Biophys Res Commun 390:931-6 (2009). ICC/IF ; Human . Read more (PubMed: 19852942) »

See all 5 Publications for this product

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There was error on the datasheet which is being updated. The antibody has been tested for ICC/IF and is fully guaranteed. I am sorry 100% Abreview discount cannot be provided this time. However if you are intereste...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"