製品の概要

  • 製品名Anti-Cytokeratin 18 antibody [DC10]
    Cytokeratin 18 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [DC10] to Cytokeratin 18
  • 特異性We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human breast cancer PMC 42 cells.

  • ポジティブ・コントロール
    • HT29 cells. Skin. Breast or lung carcinoma.
  • 特記事項


    ab80572 recognizes a variety of simple epithelia including glandular epithelium but not stratified squamous epithelia.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab80572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 48 kDa.

Use at a concentration of 1 - 2 µg/ml for 2hrs at RT.

IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use at a concentration of 1 - 2 µg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • 組織特異性Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • 関連疾患Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • 配列類似性Belongs to the intermediate filament family.
  • 翻訳後修飾Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • 細胞内局在Cytoplasm > perinuclear region.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Anti-Cytokeratin 18 antibody [DC10] 画像

  • Formalin-fixed, paraffin-embedded human breast cancer tissue stained with ab80572 at 2µg/ml using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.

Anti-Cytokeratin 18 antibody [DC10] (ab80572) 使用論文

This product has been referenced in:
  • Lauerová L  et al. Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma 7:495-504 (1988). Read more (PubMed: 2461901) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"