製品の概要

  • 製品名Anti-Cytokeratin 18 antibody [DA-7] (Biotin)
    Cytokeratin 18 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [DA-7] to Cytokeratin 18 (Biotin)
  • 標識Biotin
  • 特異性ab27549 recognises Cytokeratin peptide 18. We have data to indicate that this antibody may not cross react with wide range of other species. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: WB, ELISA, ICC, IHC-P, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human breast carcinoma cell line PMC-42.

  • ポジティブ・コントロール
    • Western blot and immunocytochemistry: HeLa human cervix carcinoma cell line, MCF-7 human breast adenocarcinoma cell line. Immunohistochemistry: epithelial tissue (skin). ELISA: MCF-7 human breast adenocarcinoma cell line

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab27549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Predicted molecular weight: 45 kDa.
ELISA Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

ターゲット情報

  • 機能Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • 組織特異性Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • 関連疾患Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • 配列類似性Belongs to the intermediate filament family.
  • 翻訳後修飾Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • 細胞内局在Cytoplasm > perinuclear region.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Anti-Cytokeratin 18 antibody [DA-7] (Biotin) 画像

  • Immunocytochemistry/Immunofluorescence analysis of human ovarian tumour tissue labelling Cytokeratin 18 with ab7549 conjugated to conjugated to QD655 at a molar ratio of 6:1 (Ab:QD). Tissue was incubated with primary antibody overnight at 4°C. Right image - tissue was incubated a further 2 hours at room temperature in order to remove background staining.

Anti-Cytokeratin 18 antibody [DA-7] (Biotin) (ab27549) 使用論文

This product has been referenced in:
  • Sweeney E  et al. Quantitative multiplexed quantum dot immunohistochemistry. Biochem Biophys Res Commun 374:181-6 (2008). ICC ; Human . Read more (PubMed: 18621021) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"