Biotin Anti-Cytokeratin 18 抗体 [C-04] (ab27553)
Key features and details
- Biotin Mouse monoclonal [C-04] to Cytokeratin 18
- Suitable for: WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Related conjugates and formulations
製品の概要
-
製品名
Biotin Anti-Cytokeratin 18 antibody [C-04]
Cytokeratin 18 一次抗体 製品一覧 -
製品の詳細
Biotin Mouse monoclonal [C-04] to Cytokeratin 18 -
由来種
Mouse -
標識
Biotin -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mammals -
免疫原
Tissue, cells or virus corresponding to Human Cytokeratin 18. Cytoskeleton preparation of epidermal carcinoma cell line A431
-
ポジティブ・コントロール
- WB: HT-29 (reducing and non-reducing conditions) cell lysate.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
-
精製度
Size exclusion -
特記事項(精製)
Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
C-04 -
アイソタイプ
IgG1 -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab27553の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/500. Predicted molecular weight: 47 kDa.
|
特記事項 |
---|
WB
1/500. Predicted molecular weight: 47 kDa. |
ターゲット情報
-
機能
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. -
組織特異性
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. -
関連疾患
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. -
細胞内局在
Cytoplasm > perinuclear region. - Information by UniProt
-
参照データベース
- Entrez Gene: 3875 Human
- Omim: 148070 Human
- SwissProt: P05783 Human
- Unigene: 406013 Human
-
別名
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
- Cell proliferation-inducing gene 46 protein antibody
see all
画像
-
All lanes : Biotin Anti-Cytokeratin 18 antibody [C-04] (ab27553) at 2 µg/ml
Lane 1 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (reducing conditions)
Lane 2 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (reducing conditions)
Lane 3 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (non-reducing conditions)
Lane 4 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (non-reducing conditions)
Secondary
All lanes : Conjugated Streptavidin
Predicted band size: 47 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?Proteolytic fragment observed at approximately 25 kDa.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab27553 は 4 報の論文で使用されています。
- Gao Q et al. Paeoniflorin prevents postoperative peritoneal adhesion formation in an experimental rat model. Oncotarget 8:93899-93911 (2017). PubMed: 29212197
- Prater MD et al. Mammary stem cells have myoepithelial cell properties. Nat Cell Biol 16:942-50 (2014). IHC-P ; Mouse . PubMed: 25173976
- Kim S et al. FGFR2 promotes breast tumorigenicity through maintenance of breast tumor-initiating cells. PLoS One 8:e51671 (2013). IHC-P ; Mouse . PubMed: 23300950
- Drobinskaya I et al. Scalable selection of hepatocyte- and hepatocyte precursor-like cells from culture of differentiating transgenically modified murine embryonic stem cells. Stem Cells 26:2245-56 (2008). ICC/IF . PubMed: 18556507