製品の概要

  • 製品名Anti-Cytokeratin 18 antibody
    Cytokeratin 18 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cytokeratin 18
  • アプリケーション適用あり: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    A synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 18

  • ポジティブ・コントロール
    • A431 cell lysate, human urothelial carcinoma cells and HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP 1/100.
Flow Cyt 1/15. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.

ターゲット情報

  • 機能Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • 組織特異性Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • 関連疾患Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • 配列類似性Belongs to the intermediate filament family.
  • 翻訳後修飾Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • 細胞内局在Cytoplasm > perinuclear region.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Anti-Cytokeratin 18 antibody 画像

  • Anti-Cytokeratin 18 antibody (ab52948) at 1/50000 dilution + A431 cell lysate at 10 µg

    Secondary
    goat anti-rabbit HR at 1/2000 dilution

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • Anti-Cytokeratin 18 antibody (ab52948) at 1/250 dilution + Recombinant Human Cytokeratin 18 protein (ab73638) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute
  • ab52948 at 1/250 dilution staining Cytokeratin 18 in urothelial carcinoma cells by Immunohistochemistry, Paraffin embedded tissue.
  • ab52948 at 1/250 dilution staining Cytokeratin 18 in HeLa cells by Immunofluorescence

Anti-Cytokeratin 18 antibody (ab52948) 使用論文

This product has been referenced in:
  • Joiner AM  et al. Primary Cilia on Horizontal Basal Cells Regulate Regeneration of the Olfactory Epithelium. J Neurosci 35:13761-72 (2015). IHC ; Mouse . Read more (PubMed: 26446227) »
  • Chung CY  et al. Progressive Proximal-to-Distal Reduction in Expression of the Tight Junction Complex in Colonic Epithelium of Virally-Suppressed HIV+ Individuals. PLoS Pathog 10:e1004198 (2014). WB ; Human . Read more (PubMed: 24968145) »

See all 7 Publications for this product

Product Wall

Application ELISA
Sample Human Recombinant protein (Human Cytokeratin 18 full length protein (ab73638))
Specification Human Cytokeratin 18 full length protein (ab73638)
Type Sandwich (Capture)
Blocking step StartingBlock blocking buffer (Thermo Scientific) as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Feb 25 2015

The cat number is ab133272 and this one has been used for WB and IP.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"