製品の概要

  • 製品名Anti-Cytokeratin 15 antibody [LHK15]
    Cytokeratin 15 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [LHK15] to Cytokeratin 15
  • アプリケーション適用あり: ICC/IF, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    17 amino acids synthetic peptide derived from the C terminal region of Human Cytokeratin 15

  • ポジティブ・コントロール
    • IHC-P: FFPE human breast normal. ICC-IF: A431 cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 10mM PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノモノクローナル
  • クローン名LHK15
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 組織特異性Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate.
  • 配列類似性Belongs to the intermediate filament family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI528832 antibody
    • CK 15 antibody
    • CK-15 antibody
    • CK15 antibody
    • Cytokeratin-15 antibody
    • Cytokeratin15 antibody
    • K15 antibody
    • K1C15_HUMAN antibody
    • K1CO antibody
    • Ka15 antibody
    • Keratin 15 antibody
    • Keratin 15 basic antibody
    • Keratin 15 beta antibody
    • Keratin antibody
    • Keratin complex 1 acidic gene 15 antibody
    • Keratin type I cytoskeletal 15 antibody
    • Keratin-15 antibody
    • Keratin15 antibody
    • KRT 15 antibody
    • Krt1-15 antibody
    • KRT15 antibody
    • KRTB antibody
    • KRTL15 antibody
    • Type I cytoskeletal 15 antibody
    • Type I keratin Ka15 antibody
    see all

Anti-Cytokeratin 15 antibody [LHK15] 画像

  • IHC image of Cytokeratin 15 staining in a formalin fixed, paraffin embedded normal human breast tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80522, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • ab80522 stained in A431cells. The cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1 hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab80522 at 5 µg/ml and ab6046 (Rabbit polyclonal to beta tubulin) at 1ug/ml overnight at +4°C. The secondary antibodies were ab150117 (colored green) used at 1 ug/ml and ab150087 (pseudo-colored red) used at 2 µg/ml for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1 hour at room temperature.

  • ab80522 staining Cytokeratin 15 in mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (undiluted) for 10 hours.

    See Abreview

  • ab8969 staining keratin 15 in rat hair folicles by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in 0.25% Triton X-100 prior to blocking in 1% BSA for 10 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 21°C. A TRITC conjugated mouse anti-mouse antibody, diluted 1/85, was used as the secondary.

    See Abreview

  • Overlay histogram showing A431 cells stained with ab80522 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80522, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Cytokeratin 15 antibody [LHK15] (ab80522) 使用論文

This product has been referenced in:
  • Soady KJ  et al. Mouse mammary stem cells express prognostic markers for triple-negative breast cancer. Breast Cancer Res 17:31 (2015). ICC/IF ; Mouse . Read more (PubMed: 25849541) »
  • Waseem A  et al. Keratin 15 expression in stratified epithelia: downregulation in activated keratinocytes. J Invest Dermatol 112:362-9 (1999). WB, ICC/IF ; Human . Read more (PubMed: 10084315) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated
Sample Mouse Tissue sections (Colon)
Specification Colon
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 5%
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 23 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (hair follicle, HR)
Specification hair follicle, HR
Fixative Paraformaldehyde
Permeabilization Yes - 0.25% Triton X-100
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Maciej Gagat

Verified customer

投稿 Aug 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"