Anti-Cytokeratin 15 抗体 [EPR1614Y] (ab52816)




Our Abpromise guarantee covers the use of ab52816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/50 - 1/100.
WB 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 組織特異性Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate.
    • 配列類似性Belongs to the intermediate filament family.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AI528832 antibody
      • CK 15 antibody
      • CK-15 antibody
      • CK15 antibody
      • Cytokeratin-15 antibody
      • Cytokeratin15 antibody
      • K15 antibody
      • K1C15_HUMAN antibody
      • K1CO antibody
      • Ka15 antibody
      • Keratin 15 antibody
      • Keratin 15 basic antibody
      • Keratin 15 beta antibody
      • Keratin antibody
      • Keratin complex 1 acidic gene 15 antibody
      • Keratin type I cytoskeletal 15 antibody
      • Keratin-15 antibody
      • Keratin15 antibody
      • KRT 15 antibody
      • Krt1-15 antibody
      • KRT15 antibody
      • KRTB antibody
      • KRTL15 antibody
      • Type I cytoskeletal 15 antibody
      • Type I keratin Ka15 antibody
      see all

    Anti-Cytokeratin 15 antibody [EPR1614Y] 画像

    • Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816) at 1/10000 dilution + fetal thymus lysate at 10 µg

      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 45 kDa
      Observed band size : 45 kDa
    • ab52816 at 1/100 dilution staining Cytokeratin 15 in human squamous cervical carcinoma by Immunohistochemistry, Paraffin embedded tissue.
    • ab52816 at 1/50 dilution staining Cytokeratin 15 in HeLa cells by Immunocytochemistry.
    • Overlay histogram showing A431 cells stained with ab52816 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52816,1/100 dilution ) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.

    • Fluorescent immunohistochemical analysis of paraffin-embedded human normal skin tissue using ab52816. Green-CK15 red-PI

    • Fluorescent immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue using ab52816. Green-CK15 red-PI.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816) 使用論文

    This product has been referenced in:
    • Pal SK  et al. The expression profiles of acidic epithelial keratins in ameloblastoma. Oral Surg Oral Med Oral Pathol Oral Radiol 115:523-31 (2013). IHC-P ; Mouse, Human . Read more (PubMed: 23522646) »
    • Tai G  et al. Cytokeratin 15 marks basal epithelia in developing ureters and is upregulated in a subset of urothelial cell carcinomas. PLoS One 8:e81167 (2013). IHC-P ; Mouse . Read more (PubMed: 24260555) »

    See all 7 Publications for this product

    Product Wall

    Thank you for your reply.

    I have to confirm that we did not test this antibody in IF with paraffin embedded sections.

    This is in theory possible. Although often high background occurs with this method.

    Please follow this l...

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    Thank you for your inquiry.

    I am happy to confirm that both antibodies are tested and guarantee for IHC-P.

    1.) We used ab52816 successfully in a dilution of 1/100 in IHC-P with human squamous cervical carcinoma by Immunohistochemist...

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    Thank you for again confirming these details and for your cooperation.   As requested, I have issued a free of charge replacement with the order number xxxx. To check the status of the order please contact our Customer Service team and reference th...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has ca...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (skin section)
    Specification skin section
    Fixative Methanol
    Permeabilization Yes - acetone
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C

    Ms. Cinzia Chinnici

    Verified customer

    投稿 Aug 20 2009