リコンビナント
RabMAb

Anti-Cytokeratin 15 抗体 [EPR1614Y] (ab52816)

製品の概要

  • 製品名
    Anti-Cytokeratin 15 antibody [EPR1614Y]
    Cytokeratin 15 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1614Y] to Cytokeratin 15
  • アプリケーション
    適用あり: ICC/IF, WB, Flow Cyt, IHC-Pmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to residues from the C-terminus of human Cytokeratin 15.

  • ポジティブ・コントロール
    • Fetal thymus lysate, human squamous cervical carcinoma, and HeLa cells.
  • 特記事項

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/50 - 1/100.
WB 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    • 組織特異性
      Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate.
    • 配列類似性
      Belongs to the intermediate filament family.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AI528832 antibody
      • CK 15 antibody
      • CK-15 antibody
      • CK15 antibody
      • Cytokeratin-15 antibody
      • Cytokeratin15 antibody
      • K15 antibody
      • K1C15_HUMAN antibody
      • K1CO antibody
      • Ka15 antibody
      • Keratin 15 antibody
      • Keratin 15 basic antibody
      • Keratin 15 beta antibody
      • Keratin antibody
      • Keratin complex 1 acidic gene 15 antibody
      • Keratin type I cytoskeletal 15 antibody
      • Keratin-15 antibody
      • Keratin15 antibody
      • KRT 15 antibody
      • Krt1-15 antibody
      • KRT15 antibody
      • KRTB antibody
      • KRTL15 antibody
      • Type I cytoskeletal 15 antibody
      • Type I keratin Ka15 antibody
      see all

    画像

    • Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816) at 1/10000 dilution + fetal thymus lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 45 kDa
      Observed band size : 45 kDa
    • ab52816 at 1/100 dilution staining Cytokeratin 15 in human squamous cervical carcinoma by Immunohistochemistry, Paraffin embedded tissue.
    • ab52816 at 1/50 dilution staining Cytokeratin 15 in HeLa cells by Immunocytochemistry.
    • Overlay histogram showing A431 cells stained with ab52816 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52816,1/100 dilution ) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.

    • Fluorescent immunohistochemical analysis of paraffin-embedded human normal skin tissue using ab52816. Green-CK15 red-PI

    • Fluorescent immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue using ab52816. Green-CK15 red-PI.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    参考文献

    This product has been referenced in:
    • Gouveia RM  et al. Controlling the 3D architecture of Self-Lifting Auto-generated Tissue Equivalents (SLATEs) for optimized corneal graft composition and stability. Biomaterials 121:205-219 (2017). IF ; Human . Read more (PubMed: 28092777) »
    • Wang P  et al. Basic fibroblast growth factor reduces scar by inhibiting the differentiation of epidermal stem cells to myofibroblasts via the Notch1/Jagged1 pathway. Stem Cell Res Ther 8:114 (2017). Flow Cyt ; Rat . Read more (PubMed: 28511663) »

    See all 11 Publications for this product

    レビューと Q&A

    Thank you for your reply.

    I have to confirm that we did not test this antibody in IF with paraffin embedded sections.

    This is in theory possible. Although often high background occurs with this method.

    Please follow this l...

    Read More

    Thank you for your inquiry.

    I am happy to confirm that both antibodies are tested and guarantee for IHC-P.

    1.) We used ab52816 successfully in a dilution of 1/100 in IHC-P with human squamous cervical carcinoma by Immunohistochemist...

    Read More

    Thank you for again confirming these details and for your cooperation.   As requested, I have issued a free of charge replacement with the order number xxxx. To check the status of the order please contact our Customer Service team and reference th...

    Read More

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has ca...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (skin section)
    Specification
    skin section
    Fixative
    Methanol
    Permeabilization
    Yes - acetone
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
    Username

    Ms. Cinzia Chinnici

    Verified customer

    投稿 Aug 20 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録