Anti-Cytokeratin 14 抗体 [RCK107] (ab9220)

製品の概要

  • 製品名Anti-Cytokeratin 14 antibody [RCK107]
    Cytokeratin 14 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [RCK107] to Cytokeratin 14
  • 特異性This antibody reacts exclusively with cytokeratin 14 which is present in basal cell compartments of stratified and combined epithelia.
  • アプリケーション適用あり: Dot blot, ICC, IHC-Fr, WB, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Rat, Dog, Human, Pig
  • 免疫原

    Cytoskeletal preparation of TR146 epithelial cells.

  • ポジティブ・コントロール
    • cytoskeletal preparation of TR146 epithelial cells, skin tissue lysate (Human)
  • 特記事項


    Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • 一次抗体 備考Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
  • ポリ/モノモノクローナル
  • クローン名RCK107
  • ミエローマSp2/0-Ag14
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot 1/100 - 1/1000.
ICC Use at an assay dependent concentration.
IHC-Fr 1/100 - 1/200.
WB 1/100 - 1/1000.
IHC-P 1/50 - 1/7000. PubMed: 19812262

Heat retrieval (PubMed 19812262) and Proteinase K ()

Flow Cyt 1/100 - 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
  • 組織特異性Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
  • 関連疾患Defects in KRT14 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
    Defects in KRT14 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
    Defects in KRT14 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, although it is less severe.
    Defects in KRT14 are the cause of epidermolysis bullosa simplex autosomal recessive (AREBS) [MIM:601001]. AREBS is an intraepidermal epidermolysis bullosa characterized by localized blistering on the dorsal, lateral and plantar surfaces of the feet.
    Defects in KRT14 are the cause of Naegeli-Franceschetti-Jadassohn syndrome (NFJS) [MIM:161000]; also known as Naegeli syndrome. NFJS is a rare autosomal dominant form of ectodermal dysplasia. The cardinal features are absence of dermatoglyphics (fingerprints), reticular cutaneous hyperpigmentation (starting at about the age of 2 years without a preceding inflammatory stage), palmoplantar keratoderma, hypohidrosis with diminished sweat gland function and discomfort provoked by heat, nail dystrophy, and tooth enamel defects.
    Defects in KRT14 are the cause of dermatopathia pigmentosa reticularis (DPR) [MIM:125595]. DPR is a rare ectodermal dysplasia characterized by lifelong persistent reticulate hyperpigmentation, noncicatricial alopecia, and nail dystrophy.
  • 配列類似性Belongs to the intermediate filament family.
  • 細胞内局在Cytoplasm. Nucleus. Expressed in both as a filamentous pattern.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CK 14 antibody
    • CK-14 antibody
    • ck14 antibody
    • Cytokeratin 14 antibody
    • Cytokeratin-14 antibody
    • Cytokeratin14 antibody
    • Dowling Meara antibody
    • EBS3 antibody
    • EBS4 antibody
    • Epidermolysis bullosa simplex antibody
    • K14 antibody
    • K1C14_HUMAN antibody
    • Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner) antibody
    • Keratin 14 antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 14 antibody
    • Keratin, type I cytoskeletal 14 antibody
    • Keratin-14 antibody
    • Keratin14 antibody
    • Koebner antibody
    • Krt 14 antibody
    • Krt14 antibody
    • NFJ antibody
    • OTTHUMP00000164624 antibody
    • type I cytoskeletal 14 antibody
    see all

Anti-Cytokeratin 14 antibody [RCK107] 画像

  • ICC/IF image of ab9220 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9220, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Cytokeratin 14 antibody [RCK107] (ab9220) at 4 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • Immunohistochemistry analysis on frozen section of swine skin labeling Cytokeratin 14 with ab9220. Showing staining of basal cells.

Anti-Cytokeratin 14 antibody [RCK107] (ab9220) 使用論文

This product has been referenced in:
  • Pastar I  et al. Interactions of methicillin resistant Staphylococcus aureus USA300 and Pseudomonas aeruginosa in polymicrobial wound infection. PLoS One 8:e56846 (2013). IHC-P ; Pig . Read more (PubMed: 23451098) »
  • Lim YY  et al. Epigenetic modulation of the miR-200 family is associated with transition to a breast cancer stem-cell-like state. J Cell Sci 126:2256-66 (2013). ICC/IF ; Human . Read more (PubMed: 23525011) »

See all 10 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Mouse Tissue sections (skin)
Specification skin
Permeabilization Yes - tritonx-100
Fixative Paraformaldehyde
Username

Ms. Megan Scott

Verified customer

投稿 Aug 27 2013

Gracias por contactarnos.

Como bien dices, tenemos varios anticuerpos contra los marcadores especificados, sin embargo, que se hayan testado y validado (y por tanto, estén cubiertos por la garantía Abpromise) en inmunocitoqu&iac...

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Thank you for your email and the feedback. I am glad to hear that some of our antibodies work very well for you. As for the 2 antibodies you mentioned that did not work as expected (ab38890 and ab9220), could you please provide a few more protocol ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Skin)
Specification Skin
Fixative Acetone
Permeabilization No
Blocking step Casein as blocking agent for 10 minute(s) · Concentration: 0.25µg/mL · Temperature: 20°C
Username

Dr. Vaughan Feisst

Verified customer

投稿 Jul 20 2011

I'm sorry to hear you are having a problem with ab9220 and ab18389. I was able to find out that the antibody ab18389 has been used in a paper by Ishida-Yamamoto A et al. 1991. Invest Derm. 97 (6); 959 where a detailed protocol is described, unfortun...

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