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Cytoskeletal preparation of TR146 epithelial cells.
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Our Abpromise guarantee covers the use of ab9220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||1/100 - 1/1000.|
|ICC||Use at an assay dependent concentration.|
|IHC-Fr||1/100 - 1/200.|
|WB||1/100 - 1/1000.|
|IHC-P||1/50 - 1/7000. PubMed: 19812262
Heat retrieval (PubMed 19812262) and Proteinase K ()
|Flow Cyt||1/100 - 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
Immunohistochemistry analysis on frozen section of swine skin labeling Cytokeratin 14 with ab9220. Showing staining of basal cells.