Anti-Cytokeratin 13 抗体 [EPR3671] (ab92551)


  • 製品名Anti-Cytokeratin 13 antibody [EPR3671]
    Cytokeratin 13 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3671] to Cytokeratin 13
  • アプリケーション適用あり: IHC-Fr, WB, ICC, IHC-P, Flow Cytmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (Human)

  • ポジティブ・コントロール
    • WB: A431, HACAT or 293T cell lysate. IHC: transitional cell urinary bladder carcinoma tissue. FC: A431 cells
  • 特記事項


    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab92551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
WB 1/100000 - 1/500000. Predicted molecular weight: 50 kDa.Can be blocked with Cytokeratin 13 peptide (ab180923).
ICC 1/100 - 1/250.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt 1/20.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 組織特異性Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.
    • 関連疾患Defects in KRT13 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
    • 配列類似性Belongs to the intermediate filament family.
    • 翻訳後修飾O-glycosylated; glycans consist of single N-acetylglucosamine residues.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 47 kDa cytokeratin antibody
      • CK-13 antibody
      • CK13 antibody
      • Cytokeratin 13 antibody
      • Cytokeratin-13 antibody
      • K13 antibody
      • K1C13_HUMAN antibody
      • Ka13 antibody
      • Keratin 13 antibody
      • Keratin antibody
      • keratin type I cytoskeletal 13 antibody
      • Keratin-13 antibody
      • Krt-1.13 antibody
      • Krt1-13 antibody
      • KRT13 antibody
      • MGC161462 antibody
      • MGC3781 antibody
      • type I cytoskeletal 13 antibody
      • Type I keratin Ka13 antibody
      • WSN2 antibody
      see all

    Anti-Cytokeratin 13 antibody [EPR3671] 画像

    • All lanes : Anti-Cytokeratin 13 antibody [EPR3671] (ab92551) at 1/100000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : HACAT cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size : 50 kDa
    • ab92551 at 1/100 dilution staining Cytokeratin 13 in formalin-fixed, paraffin-embedded transitional cell usrinary bladder carcinoma tissue by immunohistochemistry. Detection: DAB staining.
    • ab92551 staining Cytokeratin 13 in Human vaginal epithelium tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.2% Triton in PBS and blocked with 1% fish skin gelatin for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An Alexa Fluor®594-conjugated Goat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.
    • Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling Cytokeratin 13 with purified ab92551 at 1/20 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

    • ab92551 showing positive staining in Cervical carcinoma tissue.

    • ab92551 showing positive staining in Normal tonsil squamous cells tissue.

    • ab92551 showing negative staining in Glioma tissue.

    • ab92551 showing negative staining in Breast carcinoma tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-Cytokeratin 13 antibody [EPR3671] (ab92551) 使用論文

    This product has been referenced in:
    • Liu S  et al. Keratin 13 Is Enriched in Prostate Tubule-Initiating Cells and May Identify Primary Prostate Tumors that Metastasize to the Bone. PLoS One 11:e0163232 (2016). IHC ; Human . Read more (PubMed: 27711225) »
    • Chung BM  et al. Regulation of C-X-C chemokine gene expression by keratin 17 and hnRNP K in skin tumor keratinocytes. J Cell Biol 208:613-27 (2015). Read more (PubMed: 25713416) »

    See all 7 Publications for this product

    Product Wall

    Thank you for contacting us.

    This product because it is IVD has not been imported to Abcam website. Epitomics still sell this product via their website. Please contact them at the following email address;
    Read More

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (vaginal epithelium)
    Specification vaginal epithelium
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.2% Triton in PBS
    Blocking step (agent) for 30 minute(s) · Concentration: 1% · Temperature: 25°C

    Abcam user community

    Verified customer

    投稿 Oct 19 2011