製品の概要

  • 製品名
    Anti-Cytokeratin 13 antibody [AE8]
    Cytokeratin 13 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AE8] to Cytokeratin 13
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rabbit, Human
  • 免疫原

    Rabbit esophageal keratins.

  • 特記事項


    This antibody is specific for Cytokeratin 13, which is a marker for oesophageal type differentiation which is expressed by various internal stratified epithelia.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 25076852
WB 1/1000 - 1/3000. Predicted molecular weight: 50 kDa.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 組織特異性
    Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.
  • 関連疾患
    Defects in KRT13 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
  • 配列類似性
    Belongs to the intermediate filament family.
  • 翻訳後修飾
    O-glycosylated; glycans consist of single N-acetylglucosamine residues.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 47 kDa cytokeratin antibody
    • CK-13 antibody
    • CK13 antibody
    • Cytokeratin 13 antibody
    • Cytokeratin-13 antibody
    • K13 antibody
    • K1C13_HUMAN antibody
    • Ka13 antibody
    • Keratin 13 antibody
    • Keratin antibody
    • keratin type I cytoskeletal 13 antibody
    • Keratin-13 antibody
    • Krt-1.13 antibody
    • Krt1-13 antibody
    • KRT13 antibody
    • MGC161462 antibody
    • MGC3781 antibody
    • type I cytoskeletal 13 antibody
    • Type I keratin Ka13 antibody
    • WSN2 antibody
    see all

画像

  • ab16112 staining Cytokeratin 13 in Human pharynx tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in Tris pH9. Samples were incubated with primary antibody (undiluted) for 1 hour at 20°C. An undiluted HRP-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Okuda Y  et al. Wnt signaling as a possible promoting factor of cell differentiation in pleomorphic adenomas. Int J Med Sci 11:971-8 (2014). ICC/IF ; Human . Read more (PubMed: 25076852) »
  • Rival-Gervier S  et al. Kinetics and epigenetics of retroviral silencing in mouse embryonic stem cells defined by deletion of the D4Z4 element. Mol Ther 21:1536-50 (2013). Read more (PubMed: 23752310) »

See all 12 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Conjunctiva)
Antigen retrieval step
None
Permeabilization
Yes - Triton 0,3% - 5 minutesr
Specification
Conjunctiva
Blocking step
Normal Goat Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde
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投稿 Aug 21 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH9
Sample
Human Tissue sections (pharynx)
Specification
pharynx
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 29 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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