Anti-Cytokeratin 13 抗体 (ab154346)
Key features and details
- Rabbit polyclonal to Cytokeratin 13
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cytokeratin 13 antibody
Cytokeratin 13 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cytokeratin 13 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 259-458 of Human Cytokeratin 13
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ポジティブ・コントロール
- MCF7 whole cell lysate; MCF7 cells; CAL27 xenograft
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154346の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 50 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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組織特異性
Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta. -
関連疾患
Defects in KRT13 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
O-glycosylated; glycans consist of single N-acetylglucosamine residues. - Information by UniProt
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参照データベース
- Entrez Gene: 3860 Human
- Omim: 148065 Human
- SwissProt: P13646 Human
- Unigene: 654550 Human
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別名
- 47 kDa cytokeratin antibody
- CK-13 antibody
- CK13 antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded CAL27 xenograft labeling Cytokeratin 13 with ab154346 at 1/500 dilution
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Anti-Cytokeratin 13 antibody (ab154346) at 1/5000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 50 kDa
10% SDS PAGE -
Immunofluorescence analysis of methanol-fixed MCF7 cells labeling Cytokeratin 13 with ab154346 at 1/200 dilution
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154346 は 1 報の論文で使用されています。
- de Carvalho Dias K et al. Development and characterization of a 3D oral mucosa model as a tool for host-pathogen interactions. J Microbiol Methods 152:52-60 (2018). PubMed: 30017850