Anti-Cytokeratin 10 抗体 [RKSE60] (ab9025)


  • 製品名Anti-Cytokeratin 10 antibody [RKSE60]
    Cytokeratin 10 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [RKSE60] to Cytokeratin 10
  • 特異性This antibody reacts exclusively with cytokeratin 10 which is present in keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas
  • アプリケーション適用あり: Flow Cyt, IHC-Fr, ICC, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Dog, Human, Pig
    非交差種: Zebrafish
  • 免疫原

    Cytokeratins from the human epidermis.

  • ポジティブ・コントロール
    • human keratinocytes


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名RKSE60
  • ミエローマSp2/0
  • アイソタイプIgG1
  • 研究分野


Our Abpromise guarantee covers the use of ab9025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/100 - 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
WB 1/500 - 1/1000. (see reviews). Detects two bands of approximately 55 kDa (heterotetramer of two type I and two type II keratins).


  • 組織特異性Seen in all suprabasal cell layers including stratum corneum.
  • 関連疾患Defects in KRT10 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
    Defects in KRT10 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
  • 配列類似性Belongs to the intermediate filament family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BCIE antibody
    • BIE antibody
    • CK 10 antibody
    • CK-10 antibody
    • Cytokeratin-10 antibody
    • EHK antibody
    • K10 antibody
    • K1C10_HUMAN antibody
    • Keratin 10 antibody
    • Keratin 10 type I antibody
    • Keratin antibody
    • Keratin type i cytoskeletal 10 antibody
    • Keratin type I cytoskeletal 59 kDa antibody
    • Keratin-10 antibody
    • Keratin10 antibody
    • KPP antibody
    • KRT10 antibody
    • type I cytoskeletal 10 antibody
    see all

Anti-Cytokeratin 10 antibody [RKSE60] 画像

  • ab9025 staining Cytokeratin 10 in human skin tissue by Immunohistochemistry (Frozen sections). The tissue was acetone fixed and then blocked using 0.25% casein for 10 minutes at 20°C. Samples were then incubated with primary antibody at 1/1000 for 1 hour at 20°C. The secondary antibody used was a mouse anti-mouse monoclonal conjugated to Alexa Fluor® 488 (green) used at a 1/200 dilution.

    See Abreview

  • Immunohistochemistry on frozen section of swine skin: positive staining of the keratinizing layer
  • Immunohistochemistry on paraffin section of human skin
  • Immunofluorescence staining of a 7 days old zebrafish embryo

Anti-Cytokeratin 10 antibody [RKSE60] (ab9025) 使用論文

This product has been referenced in:
  • Tabuchi Y  et al. Development of oral epithelial cell line ROE2 with differentiation potential from transgenic rats harboring temperature-sensitive simian virus40 large T-antigen gene. Exp Anim 63:31-44 (2014). WB ; Rat . Read more (PubMed: 24521861) »
  • Zhu XJ  et al. BMP-FGF signaling axis mediates Wnt-induced epidermal stratification in developing mammalian skin. PLoS Genet 10:e1004687 (2014). IHC ; Mouse . Read more (PubMed: 25329657) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Pig Tissue lysate - whole (skin)
Gel Running Conditions Reduced Denaturing
Loading amount 25 µg
Treatment adipose-derived stem cell culture media
Specification skin
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Jul 29 2016

Application Western blot
Sample Pig Tissue lysate - whole (skin)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Treatment burn wound with stem cell treatment
Specification skin
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

投稿 May 20 2015

Vous avez testé l'anticorps anti-cytokeratin 10 et l'isotype en Western Blot. Avez-vous également utilisé l'anticorps secondaire seul?
Je suis étonné que la bande observée avec l'isotype se retrouve au m&ec...

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Mercipour votre réponse ainsi que pour le questionnaire rempli et détaillé.

Il m'est toujours impossible de trouver une explication définitive à votre problème.
Est-ce que l'anticorps anti-K10 est ...

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En plus de vos résultats obtenus en Western Blot, vous serait-il possible de remplir et nous retourner le questionnaire ci-joint?

Les informations sur le protocole utilisé nous sera utile pour tenter de comprendre le problè...

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Merci de nous avoir contactés.

Je vous envoie comme convenu un email auquel vous pourrez répondre et joindre vos résultats en Western Blot avec les anticorps ab9025 et ab18448.
Votre réponse me sera alors automa...

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Le laboratoire vient de nous confirmer que la chaine légère du clone [RKSE60] est de type kappa.

Thank you for your phone call. I found just the information in our records and am happy to confirm that the laboratory has obtained good results with this antibody at 1:100 – 1:200 for flow cytometry. I hope this information is helpful. Please do no...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Skin)
Specification Skin
Fixative Acetone
Permeabilization No
Blocking step Casein as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 20°C

Dr. Vaughan Feisst

Verified customer

投稿 Sep 17 2010

Thank you for contacting us for technical support. I do not advise the customer to use this antibody on mouse tissue in immunohistochemical methods as it is raised in mouse. Therefore the secondary anti-mouse antibody will bind to the whole of the t...

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