Anti-Cytokeratin 10 抗体 [DE-K10] (ab9026)

製品の概要

  • 製品名
    Anti-Cytokeratin 10 antibody [DE-K10]
    Cytokeratin 10 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [DE-K10] to Cytokeratin 10
  • 特異性
    Reacts with keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas.
  • アプリケーション
    適用あり: IHC-Fr, ICC, IHC-P, WB, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Sheep, Cat, Dog, Human
  • 免疫原

    Cytoskeletal preparation extracted from human epidermis

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    DE-K10
  • ミエローマ
    Sp2/0
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P 1/100.
WB 1/100 - 1/1000.
Flow Cyt 1/100 - 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 組織特異性
    Seen in all suprabasal cell layers including stratum corneum.
  • 関連疾患
    Defects in KRT10 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
    Defects in KRT10 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
  • 配列類似性
    Belongs to the intermediate filament family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BCIE antibody
    • BIE antibody
    • CK 10 antibody
    • CK-10 antibody
    • Cytokeratin-10 antibody
    • EHK antibody
    • K10 antibody
    • K1C10_HUMAN antibody
    • Keratin 10 antibody
    • Keratin 10 type I antibody
    • Keratin antibody
    • Keratin type i cytoskeletal 10 antibody
    • Keratin type I cytoskeletal 59 kDa antibody
    • Keratin-10 antibody
    • Keratin10 antibody
    • KPP antibody
    • KRT10 antibody
    • type I cytoskeletal 10 antibody
    see all

Anti-Cytokeratin 10 antibody [DE-K10] 画像

  • IHC image on a frozen section of  dog skin showing its strong reactivity in the keratinizing epidermal cells.

  • IHC image of ab9026 staining in Human cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9026, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Mesenchymal-epidermal interactions assessed by heterologous organotypic co-cultures (OTC). Heterologous OTC consisting of mice deficient for cathepsin L +/+ fibroblasts in collagen type I gels topped by normal human primary keratinocytes were grown air-exposed for 7 days. Paraffin sections were stained in haematoxylin and eosin (HE) or by immunohistochemistry for the proliferation marker Ki67 (Ki67, brown nuclear staining) and the differentiation markers cytokeratin 10 (K10, ab9026 1/100 dilution, brown staining) and transglutaminase (TG, brown staining). Bar 100 µm.

Anti-Cytokeratin 10 antibody [DE-K10] (ab9026) 使用論文

This product has been referenced in:
  • Furukawa S  et al. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 30:79-107 (2017). IHC ; Mouse . Read more (PubMed: 28190929) »
  • Wang P  et al. Basic fibroblast growth factor reduces scar by inhibiting the differentiation of epidermal stem cells to myofibroblasts via the Notch1/Jagged1 pathway. Stem Cell Res Ther 8:114 (2017). Flow Cyt ; Rat . Read more (PubMed: 28511663) »

See all 18 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (SKIN)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA PH9
Permeabilization
Yes - 0,05% TX 100
Specification
SKIN
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde
Username

Dr. Vincenzo Miragliotta

Verified customer

投稿 Jun 05 2017

Application
Western blot
Sample
Pig Tissue lysate - whole (skin)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
nurn wound with stem cell treatment
Specification
skin
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 May 20 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.05% typsin
Sample
Human Tissue sections (skin)
Specification
skin
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 23 2015

I have just received the following information from the supplier of ab9026: "1) Yes please, you can offer your customer a free (replacement) batch of our other keratin 10 ab9025. 2)The question is however, whether this will be of any help. Ab902...

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I'm sorry to hear you are having problem with ab9026. Spotting can be frequently caused by the blocking agent you use, I would therefore try filtering it and leaving on the membrane for 30min, then rinsing the membrane with TBST. Incubate the prima...

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The exact epitope of Ab9026 is not known. I'm afraid you just have to try and see if your truncated protein is still recognized by Ab9026.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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