製品の概要

  • 製品名Anti-Cytokeratin 10 antibody
    Cytokeratin 10 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cytokeratin 10
  • アプリケーション適用あり: WB, IHC-Fr, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 23 and 284 of Human Cytokeratin 10 (NP_000412).

  • ポジティブ・コントロール
    • A431 cell lysate.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab97764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 59 kDa.
IHC-Fr 1/100.
IP Use a concentration of 5 µg/ml.

ターゲット情報

  • 組織特異性Seen in all suprabasal cell layers including stratum corneum.
  • 関連疾患Defects in KRT10 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
    Defects in KRT10 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
  • 配列類似性Belongs to the intermediate filament family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BCIE antibody
    • BIE antibody
    • CK 10 antibody
    • CK-10 antibody
    • Cytokeratin-10 antibody
    • EHK antibody
    • K10 antibody
    • K1C10_HUMAN antibody
    • Keratin 10 antibody
    • Keratin antibody
    • Keratin type i cytoskeletal 10 antibody
    • Keratin type I cytoskeletal 59 kDa antibody
    • Keratin-10 antibody
    • Keratin10 antibody
    • KPP antibody
    • KRT10 antibody
    • type I cytoskeletal 10 antibody
    see all

Anti-Cytokeratin 10 antibody 画像

  • Anti-Cytokeratin 10 antibody (ab97764) at 1/3000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 59 kDa
    7.5% SDS PAGE
  • ab97764 staining Cytokeratin 10 in human skin tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in acetone, blocked using 5% serum for 20 minutes at 20°C and then incubated with ab97764 at a 1/100 dilution for 30 minutes at 20°C. The secondary used was an Alexa-Fluor 488 conjugated goat polyclonal used at a 1/200 dilution.

    See Abreview

  • Cytokeratin 10 was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to Cytokeratin 10 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab97764.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 59kDa; Cytokeratin 10, non specific bands - 52kDa and in control (lane 2): We are unsure as to the identity of this extra band.

Anti-Cytokeratin 10 antibody (ab97764) 使用論文

This product has been referenced in:
  • Suga H  et al. Tracking the elusive fibrocyte: identification and characterization of collagen-producing hematopoietic lineage cells during murine wound healing. Stem Cells 32:1347-60 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24446236) »
  • Jiang F  et al. Serum Proteome Changes in Healthy Subjects with Different Genotypes of NOS1AP in the Chinese Population. J Diabetes Res 2013:357630 (2013). WB ; Human . Read more (PubMed: 23671866) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Skin)
Specification Skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Nov 09 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Skin)
Specification Skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Nov 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Apr 02 2012

Code de remise : *************** Date d’expiration : JJ MM AAAA Nous sommes ravis que vous souhaitiez participer à notre offre et tester ab97764 en IHC-Fr. Le code de réduction correspondant est *****. Ce code de réduction est utilisable sous 120...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"