製品の概要

  • 製品名Anti-Cytokeratin 10 antibody
    Cytokeratin 10 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cytokeratin 10
  • 特異性ab53124 detects endogenous levels of total Cytokeratin 10 protein.
  • アプリケーション適用あり: IHC-P, ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    A synthetic peptide derived from human Cytokeratin 10.

  • ポジティブ・コントロール
    • HeLa cell extracts.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab53124 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab53124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.
WB 1/300 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ELISA 1/5000.

ターゲット情報

  • 組織特異性Seen in all suprabasal cell layers including stratum corneum.
  • 関連疾患Defects in KRT10 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
    Defects in KRT10 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
  • 配列類似性Belongs to the intermediate filament family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BCIE antibody
    • BIE antibody
    • CK 10 antibody
    • CK-10 antibody
    • Cytokeratin-10 antibody
    • EHK antibody
    • K10 antibody
    • K1C10_HUMAN antibody
    • Keratin 10 antibody
    • Keratin antibody
    • Keratin type i cytoskeletal 10 antibody
    • Keratin type I cytoskeletal 59 kDa antibody
    • Keratin-10 antibody
    • Keratin10 antibody
    • KPP antibody
    • KRT10 antibody
    • type I cytoskeletal 10 antibody
    see all

Anti-Cytokeratin 10 antibody 画像

  • All lanes : Anti-Cytokeratin 10 antibody (ab53124) at 1/300 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with immunising peptide


    Predicted band size : 60 kDa
    Observed band size : 60 kDa
  • Immunohistochemical analysis of Human abdominal skin tissue, staining Cytokeratin 10 with ab53124.

    Sections were blocked with 10% normal goat serum in BSA/PBS-T for 20 minutes at room temperature. Samples were incubated with primary antibody (1/1000) in BSA/PBS-T overnight at 4°C. A FITC-conjugated goat anti-rabbit IgG (1/100) was used as the secondary antibody.

Anti-Cytokeratin 10 antibody (ab53124) 使用論文

This product has been referenced in:
  • Butovich IA  et al. Biophysical and morphological evaluation of human normal and dry eye meibum using hot stage polarized light microscopy. Invest Ophthalmol Vis Sci 55:87-101 (2014). Read more (PubMed: 24282231) »
  • Kawabata K  et al. The presence of N(e)-(Carboxymethyl) lysine in the human epidermis. Biochim Biophys Acta 1814:1246-52 (2011). WB, IHC-P ; Human . Read more (PubMed: 21708295) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Skin)
Specification Skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Nov 09 2012

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Skin)
Specification Skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Nov 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (skin)
Specification skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: H-3300 Vector lab
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 01 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"