Anti-Cytokeratin 1 - 8 抗体 [AE3], prediluted (ab34255)

製品の概要

  • 製品名Anti-Cytokeratin 1 - 8 antibody [AE3], prediluted
    Cytokeratin 1 - 8 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AE3] to Cytokeratin 1 - 8, prediluted
  • 特異性ab32455 reacts with most of the Basic (Type II) keratins. Including epidermal cell keratin of 86 and 58kD and skin at 58kD, 65kD and 67kD. Broad keratin reactivity is found on human epidermal cell, oesophagus and cornea. Since each epithelium contains at least one basic keratin, AE3 is broadly reactive and stains positively almost all epithelia and their neoplasms.
  • アプリケーション適用あり: IHC-FoFr, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Chicken, Cow, Fish, Monkey, Macaque Monkey
  • 免疫原

    Tissue/ cell preparation - Human epidermal cells.

  • ポジティブ・コントロール
    • Human epidermal cells, oesophagus, cornea.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab34255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr
IHC-P
IHC-Fr
  • 追加情報IHC-Fr: Use neat
    IHC-FoFr: Use neat.
    IHC-P: Use neat. This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    Anti-Cytokeratin 1 - 8 antibody [AE3], prediluted (ab34255) 使用論文

    This product has been referenced in:
    • Tsubura A  et al. Immunophenotypic difference of keratin expression in normal mammary glandular cells from five different species. Acta Anat (Basel) 140:287-93 (1991). Read more (PubMed: 1714223) »
    • Eichner R  et al. Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression. J Cell Biol 98:1388-96 (1984). Read more (PubMed: 6201491) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Chicken Tissue sections (chicken embryo epicardium)
    Specification chicken embryo epicardium
    Fixative Paraformaldehyde
    Permeabilization Yes - 0,2% Triton X-100 in PBS, 10'
    Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Mrs. Elena Astanina

    Verified customer

    投稿 Feb 20 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"