Cytohesin 2 overexpression 293T lysate (whole cell) (ab94253)
製品の概要
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製品名
Cytohesin 2 overexpression 293T lysate (whole cell) -
特記事項
ab94253 is a 293T cell transfected lysate in which Human Cytohesin 2 has been transiently over-expressed using a pCMV-Cytohesin 2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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アプリケーション
適用あり: WBmore details
製品の特性
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Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
バッファー
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究分野
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背景
Function: Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane. Tissue specificity: Ubiquitous. Similarity: Contains 1 PH domain. Contains 1 SEC7 domain. Domain: The PH domain is necessary and sufficient for plasma membrane relocalization.
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab94253の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent dilution.
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特記事項 |
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WB
Use at an assay dependent dilution. |
画像
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ab94253 at 15μg/lane on an SDS-PAGE gel
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All lanes : Anti-Cytohesin 2 antibody (ab56510) at 1/500 dilution
Lane 1 :Cytohesin 2 overexpression 293T lysate (whole cell) (ab94253)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab94253 は論文での使用が確認できていません。