製品の概要

  • 製品名Anti-Cytohesin 2 antibody [10A12]
    Cytohesin 2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [10A12] to Cytohesin 2
  • 特異性Detects recombinant human cytohesin-2 (ARNO).
  • アプリケーション適用あり: ICC/IF, IHC-P, IPmore details
    適用なし: WB
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant full length protein (His-tag) corresponding to Cytohesin 2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/20.
IHC-P 1/25 - 1/200.
IP Use at an assay dependent concentration.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane.
    • 組織特異性Ubiquitous.
    • 配列類似性Contains 1 PH domain.
      Contains 1 SEC7 domain.
    • ドメインThe PH domain is necessary and sufficient for plasma membrane relocalization.
    • 細胞内局在Cell membrane. Cytoplasm. Both isoform 1 and isoform 2 are recruited to the cell membrane through its association with ARL4A, ARL4C and ARL4D. Requires also interaction with phosphoinositides for targeting to plasma membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ARF exchange factor antibody
      • ARF nucleotide binding site opener antibody
      • ARF nucleotide-binding site opener antibody
      • Arno antibody
      • ARNO protein antibody
      • CLM2 antibody
      • CTS18 antibody
      • CTS18.1 antibody
      • CYH2_HUMAN antibody
      • Cyth2 antibody
      • Cytohesin 2 antibody
      • Cytohesin-2 antibody
      • MGC137537 antibody
      • MGC80440 antibody
      • PH antibody
      • PH, SEC7 and coiled-coil domain-containing protein 2 antibody
      • Pleckstrin homology Sec7 and coiled coil domains 2 antibody
      • Pleckstrin homology Sec7 and coiled coil domains protein 2 antibody
      • Protein ARNO antibody
      • PSCD2 antibody
      • PSCD2L antibody
      • PSCD2L, formerly antibody
      • SEC7 and coiled-coil domain-containing protein 2 antibody
      • Sec7 antibody
      • SEC7 homolog B antibody
      • Sec7B antibody
      • SEC7L antibody
      • Sec7p L antibody
      • Sec7p-like antibody
      • Sec7pL antibody
      see all

    Anti-Cytohesin 2 antibody [10A12] 画像

    • Immunocytochemistry/Immunofluorescence analysis of Cytohesin 2 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2728 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

    • Immunocytochemistry/Immunofluorescence analysis of Cytohesin 2 in HepG2 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2728 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

    • Immunocytochemistry/Immunofluorescence analysis of Cytohesin 2 in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2728 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human colon carcinoma tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2728 (1:50) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human stomach tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2728 (1:50) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    Anti-Cytohesin 2 antibody [10A12] (ab2728) 使用論文

    ab2728 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab2728.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"