製品の概要

  • 製品名Anti-Cytoglobin antibody
    Cytoglobin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Cytoglobin
  • アプリケーション適用あり: WB, ELISA, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-191 of Human Cytoglobin

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab57713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 21 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能May have a protective function during conditions of oxidative stress. May be involved in intracellular oxygen storage or transfer.
  • 組織特異性Ubiquitously expressed. Highest expression in heart, stomach, bladder and small intestine.
  • 配列類似性Belongs to the globin family.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cygb antibody
    • CYGB_HUMAN antibody
    • Cytoglobin antibody
    • Hgb antibody
    • Histoglobin antibody
    • STAP antibody
    • Stellate cell activation associated protein antibody
    • Stellate cell activation-associated protein antibody
    see all

Anti-Cytoglobin antibody 画像



  • Predicted band size : 21 kDa
  • ab57713 at 3µg/ml, staining cytoglobin in human heart tissue by IHC-P.
  • All lanes : Anti-Cytoglobin antibody (ab57713) at 1/500 dilution

    Lane 1 : Transfected 293T lysate at 25 µg
    Lane 2 : Non-transfected 293T lysate at 25 µg


    Predicted band size : 21 kDa
  • Overlay histogram showing HeLa cells stained with ab57713 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57713, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Cytoglobin antibody (ab57713) 使用論文

ab57713 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"