Anti-Cytochrome P450 Reductase 抗体 (ab89622)
Key features and details
- Mouse polyclonal to Cytochrome P450 Reductase
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Cytochrome P450 Reductase antibody
Cytochrome P450 Reductase 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to Cytochrome P450 Reductase -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human Cytochrome P450 Reductase. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- WB: liver lysate. ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab89622の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 77 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 77 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. -
関連疾患
Defects in POR are the cause of adrenal hyperplasia variant type (AHV) [MIM:201750]; also known as Antley-Bixler syndrome-like phenotype with disordered steroidogenesis. AHV is a rare variant of congenital adrenal hyperplasia. It is an autosomal recessive disorder with apparent combined P450C17 and P450C21 deficiency. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can also present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.
Defects in POR are a cause of isolated disordered steroidogenesis (IDS) [MIM:201750]. -
配列類似性
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. -
細胞内局在
Endoplasmic reticulum membrane. Anchored to the ER membrane by its N-terminal hydrophobic region. - Information by UniProt
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参照データベース
- Entrez Gene: 5447 Human
- Omim: 124015 Human
- SwissProt: P16435 Human
- Unigene: 354056 Human
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別名
- CPR antibody
- CYPOR antibody
- Cytochrome p450 oxidoreductase antibody
see all
画像
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Anti-Cytochrome P450 Reductase antibody (ab89622) at 1 µg/ml + Human liver lysate at 50 µg
Predicted band size: 77 kDa -
All lanes : Anti-Cytochrome P450 Reductase antibody (ab89622) at 1 µg/ml
Lane 1 : Cytochrome P450 Reductase transfected 293T cell lysate
Lane 2 : Untransfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 77 kDa -
Immunofluorescence analysis of Cytochrome P450 Reductase expression in PFA-fixed HeLa cells using ab89622 at 10 µg/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab89622 は論文での使用が確認できていません。