Anti-Cytochrome P450 Reductase 抗体 (ab13513)
Key features and details
- Rabbit polyclonal to Cytochrome P450 Reductase
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Cytochrome P450 Reductase antibody
Cytochrome P450 Reductase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cytochrome P450 Reductase -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Rat, Human -
免疫原
Full length native protein (purified) corresponding to Rat Cytochrome P450 Reductase.
Database link: P00388 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Assay kits
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13513の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (3) |
1/1000. Detects a band of approximately 78 kDa.
|
ICC/IF |
Use a concentration of 10 µg/ml.
|
特記事項 |
---|
WB
1/1000. Detects a band of approximately 78 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
-
機能
This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. -
関連疾患
Defects in POR are the cause of adrenal hyperplasia variant type (AHV) [MIM:201750]; also known as Antley-Bixler syndrome-like phenotype with disordered steroidogenesis. AHV is a rare variant of congenital adrenal hyperplasia. It is an autosomal recessive disorder with apparent combined P450C17 and P450C21 deficiency. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can also present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.
Defects in POR are a cause of isolated disordered steroidogenesis (IDS) [MIM:201750]. -
配列類似性
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. -
細胞内局在
Endoplasmic reticulum membrane. Anchored to the ER membrane by its N-terminal hydrophobic region. - Information by UniProt
-
参照データベース
- Entrez Gene: 5447 Human
- Entrez Gene: 29441 Rat
- Omim: 124015 Human
- SwissProt: P16435 Human
- SwissProt: P00388 Rat
- Unigene: 354056 Human
- Unigene: 11359 Rat
-
別名
- CPR antibody
- CYPOR antibody
- Cytochrome p450 oxidoreductase antibody
see all
画像
-
Anti-Cytochrome P450 Reductase antibody (ab13513) at 1/2000 dilution + Cell lysates prepared from rat PC12 cells
Western blot analysis of rat PC12 cell lysate, probed with ab13513. -
ICC/IF image of ab13513 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13513, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (23)
ab13513 は 23 報の論文で使用されています。
- Kumondai M et al. Heterologous expression of high-activity cytochrome P450 in mammalian cells. Sci Rep 10:14193 (2020). PubMed: 32843676
- Chidawanyika T et al. A Genome-Wide CRISPR/Cas9 Screen Reveals that Riboflavin Regulates Hydrogen Peroxide Entry into HAP1 Cells. mBio 11:N/A (2020). PubMed: 32788383
- Froeling FEM et al. Bioactivation of Napabucasin Triggers Reactive Oxygen Species-Mediated Cancer Cell Death. Clin Cancer Res 25:7162-7174 (2019). PubMed: 31527169
- Yao Y et al. Effects and mechanism of amyloid ß1-42 on mitochondria in astrocytes. Mol Med Rep 17:6997-7004 (2018). PubMed: 29568933
- Pankowicz FP et al. Rapid Disruption of Genes Specifically in Livers of Mice Using Multiplex CRISPR/Cas9 Editing. Gastroenterology 155:1967-1970.e6 (2018). PubMed: 30170115